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首页> 外文期刊>European Journal of Histochemistry >Differential lectin binding patterns in the oviductal ampulla of the horse during oestrus
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Differential lectin binding patterns in the oviductal ampulla of the horse during oestrus

机译：发情期间马输卵管壶腹中不同的凝集素结合模式

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We investigated the oligosaccharide sequence of glycoconjugates, mainly sialoglycoconjugates, in the horse oviductal ampulla during oestrus by means of lectin and pre-lectin methods such as the KOH-neuraminidase procedure to remove sialic acid residues and incubation with N-glycosidase F to cleave N-linked glycans. Ciliated cells displayed Nlinked oligosaccharides throughout the cytoplasm. The cilia glycocalyx expressed both N- and O-linked (mucin-type) oligosaccharides, both showing a high variety of terminal sequences. In the most non-ciliated cells, the whole cytoplasm contained N-linked oligosaccharides with terminal aGal as well as mucin-type glycans with terminal Forssman pentasaccharides. In a few scattered non-ciliated cells, the whole cytoplasm displayed sialylated N-linked oligosaccharides with terminal Neu5Ac-GalNAc and O-linked glycans terminating with neutral and/or aGalNAc, Neu5Aca2,6Gal/ GalNAc, Neu5AcGalb1,3GalNAc. Supra-nuclear granules, probably Golgi zones, of non-ciliated cells showed mainly Olinked glycans rich in sialic acid residues. The luminal surface of non-ciliated cells showed N-linked oligosaccharides, containing terminal/internal aMan/aGlc, bGlcNAc and terminal aGal, as well as mucin-type oligosaccharides terminating with a large variety of either neutral saccharides or sialylated sequences. Apical protrusions containing O-linked oligosaccharides with terminal Forssman pentasaccharide, Neu5Ac-Galb1,4GlcNAc, Neu5Ac-GalNAc were seen in nonciliated cells scattered along the epithelium. These findings show the presence of sialoglycoconjugates in the oviductal ampulla epithelium of the mare and the existence of different lectin binding profiles between ciliated and non-ciliated (secretory) cells, as well as the presence of non-ciliated cell sub-types which might determine functional differences along the ampullary epithelium of mare oviduct.
机译：我们通过凝集素和前凝集素方法（如KOH-神经氨酸酶法）去除唾液酸残基并与N-糖苷酶F孵育以裂解N-，研究发情期间马输卵管壶腹中糖缀合物（主要是唾液酸糖缀合物）的寡糖序列。连接的聚糖。纤毛细胞在整个细胞质中均显示Nlinked寡糖。纤毛糖萼表达了N-和O-连接的（粘蛋白型）寡糖，两者均显示出多种多样的末端序列。在大多数非纤毛细胞中，整个细胞质均包含末端为aGal的N-连接寡糖以及末端为Forssman五糖的粘蛋白型聚糖。在一些散布的非纤毛细胞中，整个细胞质均显示唾液酸化的N-连接寡糖，末端带有Neu5Ac-GalNAc和O-连接的聚糖，终止于中性和/或aGalNAc，Neu5Aca2,6Gal / GalNAc，Neu5AcGalb1、3GalNAc。非纤毛细胞的超核颗粒，可能是高尔基体区域，主要显示出富含唾液酸残基的寡链聚糖。非纤毛细胞的腔表面显示出N-连接的寡糖，包含末端/内部aMan / aGlc，bGlcNAc和末端aGal，以及以多种中性糖或唾液酸化序列终止的粘蛋白型寡糖。在沿着上皮散布的非纤毛细胞中可以看到含有末端带有Forssman五糖，Nu5Ac-Galb1,4GlcNAc，Neu5Ac-GalNAc的O-连接寡糖的顶端突起。这些发现表明，母马输卵管壶腹上皮中存在唾液酸糖共轭物，纤毛和非纤毛（分泌）细胞之间存在不同的凝集素结合特性，以及纤毛非亚细胞类型的存在可能决定了沿输卵管壶腹上皮的功能差异。

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