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首页> 外文期刊>Experimental Animals >Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis
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Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis

机译：遗传表达代表性差异分析法建立与大鼠胸腺瘤易感基因连锁的多态性标记

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BUF/Mna (BUF) is a rat strain susceptible to spontaneous development of thymomas. We have previously shown that the thymoma susceptibility is controlled principally by a dominant susceptibility gene located on chromosome 7, thymoma susceptibility gene of rat 1 (Tsr1). To generate genetic markers tightly linked to Tsr1, we performed genetically directed representational difference analysis (GDRDA) with three combinations of the tester and driver DNAs. From 124 {ACI/NMs × (BUF × ACI/NMs) F1} backcross rats, 12 rats with the ACI/BUF genotype in the Tsr1 region (A/B rats) and 13 rats with the ACI/ACI genotype in the region (A/A rats) were selected, and their DNAs were pooled, respectively. Three kinds of tester DNAs, i) inbred BUF, ii) (BUF × ACI)F1, and iii) the pool from the A/B rats, were subtracted by the driver DNA prepared from the pool of the A/A rats. The three combinations yielded one, two, and one polymorphic marker(s), respectively. One marker, D7Ncc28, was isolated commonly by the three combinations of subtraction, and another marker, D11Ncc12 was isolated only by the second combination. Linkage analysis demonstrated that D7Ncc28 was located in the 8.3 cM region where Tsr1 has been mapped. The three combinations of subtraction were shown to be almost equally capable of isolating polymorphic markers in a specific chromosomal region.
机译：BUF / Mna（BUF）是对胸腺瘤自发发展敏感的大鼠品系。先前我们已经表明，胸腺瘤易感性主要是由位于大鼠7号染色体（Tsr1）的胸腺瘤易感性基因上的显性敏感性基因控制的。为了生成与Tsr1紧密相连的遗传标记，我们使用了测试仪和驱动程序DNA的三种组合进行了遗传指导的代表性差异分析（GDRDA）。从124只{ACI / NMs×（BUF×ACI / NMs）F 1 }回交大鼠中，Tsr1区具有ACI / BUF基因型的12只大鼠（A / B大鼠）和13只具有Tsr1区ACI / BUF基因型的大鼠。选择该地区（A / A大鼠）的ACI / ACI基因型，并分别收集其DNA。从库中制备的驱动程序DNA减去i）自交BUF，ii）​​（BUF×ACI）F 1 和iii）A / B大鼠库中的三种测试剂DNA。 A / A大鼠。三种组合分别产生一个，两个和一个多态性标记。一个标记D7Ncc28，通常通过减法的三种组合分离，而另一个标记D11Ncc12，仅通过第二种组合分离。连锁分析表明，D7Ncc28位于已映射Tsr1的8.3 cM区域。减法的三种组合被证明几乎能够分离特定染色体区域中的多态性标记。

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