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首页> 外文期刊>Genetics and molecular biology: publication of the Sociedade Brasileira de Genetica >Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)
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Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)

机译：新热带淡水鱼Pacu（Piaractus mesopotamicus）生长激素cDNA的分子克隆和序列分析

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摘要

RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH sequence of a Neotropical characiform fish deposited in GenBank.
机译：RT-PCR被用于扩增从垂体细胞提取的mRNA获得的Piaractus mesopotamicus生长激素（GH）cDNA。克隆扩增的片段并确定完整的cDNA序列。克隆的cDNA包含543个核苷酸的序列，该序列编码了178个氨基酸的多肽，对应于成熟的美索不达米亚GH。与其他GH序列的比较显示，在美索不动杆菌的推定多肽的N末端定位了10个氨基酸的缺口。在家庭的其他成员中也观察到同样的差距。用属于不同分类组的鱼类的GH序列进行邻域结合树分析，将美索不达米亚弓形虫GH放置在耳镜组内。据我们所知，这是在GenBank中沉积的新热带特征型鱼类的第一个GH序列。

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