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Isolation and complete genome sequencing of Mimivirus bombay , a Giant Virus in sewage of Mumbai, India

机译:印度孟买污水中的巨型病毒Mimivirus bombay的分离和完整基因组测序

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Abstract We report the isolation and complete genome sequencing of a new Mimiviridae family member, infecting Acanthamoeba castellanii, from sewage in Mumbai, India. The isolated virus has a particle size of about 435 nm and a 1,182,200-bp genome. A phylogeny based on the {DNA} polymerase sequence placed the isolate as a new member of the Mimiviridae family lineage A and was named as Mimivirus bombay. Extensive presence of Mimiviridae family members in different environmental niches, with remarkably similar genome size and genetic makeup, point towards an evolutionary advantage that needs to be further investigated. The complete genome sequence of Mimivirus bombay was deposited at GenBank/EMBL/DDBJ under the accession number KU761889.
机译:摘要我们报道了从印度孟买的污水中分离到的一个新的Mimiviridae家族成员,并感染了Acanthamoeba castellanii,并进行了完整的基因组测序。分离的病毒具有约435nm的粒径和1182200bp的基因组。基于{DNA}聚合酶序列的系统进化论将该分离物作为Mimiviridae家族谱系A的新成员,被命名为Mimivirus bombay。 Mimiviridae家族成员广泛存在于不同的环境环境中,其基因组大小和遗传组成非常相似,这表明其进化优势尚待进一步研究。 Mimivirus轰击的完整基因组序列已保藏在GenBank / EMBL / DDBJ,登录号为KU761889。

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