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Occurrence of aflatoxin in agricultural produce from local?markets in Burundi and Eastern Democratic Republic of?Congo

机译:布隆迪和刚果民主共和国东部市场的农产品中黄曲霉毒素的含量

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Aflatoxins are noxious secondary metabolites, of certain fungal species, found in food and feed. Contamination of a commodity with aflatoxins is associated with production and storage losses, and subsequently less food availability. Aflatoxins can also pose human health risks and represent a barrier to the development of trade, in both domestic and international markets. In this study, samples of cassava, maize, groundnut, beans, soybean, sorghum and milk, and their processed products were collected from local markets in Burundi and Eastern DRC. In order to investigate the levels of aflatoxin, crop samples were analyzed using a single step lateral flow immunochromatographic assay (Reveal Q+), while enzyme‐linked immune‐sorbent assay (ELISA) was used to analyze aflatoxin‐M 1 in milk, yogurt, and cheese samples. The results revealed the presence of aflatoxins in all samples from both countries, with levels ranging from 1.3 to 2,410?μg/kg. Samples collected from Burundi contained relatively higher ( p ??0.0.5) levels of aflatoxins. In 51% of all the crops samples, total aflatoxin contamination was above the EU maximum tolerable level of 4?μg/kg. Processed products, particularly from groundnut, maize, and sorghum, had the highest levels of aflatoxin contamination when compared to unprocessed grain. With regard to milk and dairy products, the level of aflatoxin‐M 1 ranged from 4.8 to 261.1?ng/kg. Approximately 29% of milk and yogurt samples had aflatoxin‐M 1 higher than the EU regulatory limit of 50?ng/kg, whereas 20% of cheese samples were found to be contaminated at levels higher than the maximum limit of 250?ng/kg. These results can serve as the basis for pre‐ and postharvest approaches to reduce aflatoxin contamination in agricultural commodities in Burundi and Eastern DRC in order to reduce health risk, avoid reduced production in livestock, and open up export markets.
机译:黄曲霉毒素是食物和饲料中某些真菌种类的有害次生代谢产物。黄曲霉毒素污染商品会导致生产和储存损失,进而导致粮食供应量减少。在国内和国际市场上,黄曲霉毒素还可能对人类健康构成威胁,并成为贸易发展的障碍。在这项研究中,从布隆迪和刚果民主共和国东部的当地市场收集了木薯,玉米,花生,豆类,大豆,高粱和牛奶及其加工产品的样品。为了研究黄曲霉毒素的水平,使用单步侧向流免疫色谱分析(Reveal Q +)对农作物样品进行了分析,而酶联免疫吸附试验(ELISA)用于分析牛奶,酸奶,和奶酪样品。结果显示,两国的所有样品中均存在黄曲霉毒素,含量范围为1.3至2,410?g / kg。从布隆迪收集的样品中黄曲霉毒素的含量较高(p≥0.0.5)。在所有农作物样品中,有51%的总黄曲霉毒素污染高于欧盟最大允许水平4?μg/ kg。与未经加工的谷物相比,经过加工的产品,尤其是花生,玉米和高粱的加工产品,其黄曲霉毒素污染水平最高。对于牛奶和奶制品,黄曲霉毒素-M 1的含量范围为4.8至261.1ng / kg。大约29%的牛奶和酸奶样品中的黄曲霉毒素-M 1高于欧盟法规规定的50?ng / kg的限值,而20%的奶酪样品中的污染水平高于最高限值250?ng / kg 。这些结果可为减少布隆迪和东部刚果(金)东部农产品中黄曲霉毒素污染的收获前和收获后方法的基础,以减少健康风险,避免牲畜减产和开放出口市场。

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