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首页> 外文期刊>Fisheries and Aquatic Sciences >Development of Polymorphic Microsatellite Markers Suitable for Genetic Linkage Mapping of Olive Flounder Paralichthys olivaceus
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Development of Polymorphic Microsatellite Markers Suitable for Genetic Linkage Mapping of Olive Flounder Paralichthys olivaceus

机译：适用于橄榄比目鱼Olivaceus基因连锁图谱的多态性微卫星标记的开发。

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Microsatellite markers are important for gene mapping and for marker-assisted selection. Sixty-five polymorphic microsatellite markers were developed with an enriched partial genomic library from olive flounder Paralichthys olivaceus an important commercial fish species in Korea. The variability of these markers was tested in 30 individuals collected from the East Sea (Korea). The number of alleles for each locus ranged from 2 to 33 (mean, 17.1). Observed and expected heterozygosity as well as polymorphism information content varied from 0.313 to 1.000 (mean, 0.788), from 0.323 to 0.977 (mean, 0.820), and from 0.277 to 0.960 (mean, 0.787), respectively. Nine loci showed significant deviation from the Hardy-Weinberg equilibrium after sequential Bonferroni correction. Analysis with MICROCHECKER suggested the presence of null alleles at five of these loci with estimated null allele frequencies of 0.126-0.285. These new microsatellite markers from genomic libraries will be useful for constructing a P. olivaceus linkage map.
机译：微卫星标记对于基因作图和标记辅助选择很重要。利用丰富的部分基因组文库，开发了65种多态性微卫星标记，这些基因组库来自橄榄比目鱼Paralichthys olivaceus，这是韩国一种重要的商业鱼类。在从东海（韩国）收集的30位个体中测试了这些标记的变异性。每个基因座的等位基因数量范围为2到33（平均值为17.1）。观察到的和预期的杂合性以及多态性信息含量分别从0.313到1.000（平均值0.788），从0.323到0.977（平均值0.820）和0.277到0.960（平均值0.787）变化。连续进行Bonferroni校正后，有9个基因座显示出与Hardy-Weinberg平衡显着偏离。用MICROCHECKER分析表明，在这些基因座中的五个基因座上存在无效等位基因，估计无效等位基因频率为0.126-0.285。来自基因组文库的这些新的微卫星标记将可用于构建橄榄假单胞菌连锁图。

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