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首页> 外文期刊>Frontiers in Public Health >Prevalence of Genotypes That Determine Resistance of Staphylococci to Macrolides and Lincosamides in Serbia
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Prevalence of Genotypes That Determine Resistance of Staphylococci to Macrolides and Lincosamides in Serbia

机译：决定葡萄球菌对大环内酯类和林可酰胺类药物耐药性的基因型在塞尔维亚的流行

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Macrolides, lincosamides, and streptogramins (MLS) resistance genes are responsible for resistance to these antibiotics in Staphylococcus infections. The purpose of the study was to analyze the distribution of the MLS resistance genes in community- and hospital-acquired Staphylococcus isolates. The MLS resistance phenotypes [constitutive resistance to macrolide–lincosamide–streptogramin B (cMLSb), inducible resistance to macrolide–lincosamide–streptogramin B (iMLSb), resistance to macrolide/macrolide–streptogramin B (M/MSb), and resistance to lincosamide–streptogramin A/streptogramin B (LSa/b)] were determined by double-disc diffusion method. The presence of the MLS resistance genes (ermA, ermB, ermC, msrA/B, lnuA, lnuB, and lsaA) were determined by end-point polymerase chain reaction in 179 isolates of staphylococci collected during 1-year period at the Center for Microbiology of Public Health Institute in Vranje. The most frequent MLS phenotype among staphylococcal isolates, both community-acquired and hospital-acquired, was iMLSb (33.4%). The second most frequent was M/MSb (17.6%) with statistically significantly higher number of hospital-acquired staphylococcal isolates (p < 0.05). MLS resistance was mostly determined by the presence of msrA/B (35.0%) and ermC (20.8%) genes. Examined phenotypes were mostly determined by the presence of one gene, especially by msrA/B (26.3%) and ermC (14.5%), but 15.6% was determined by a combination of two or more genes. M/MSb phenotype was the most frequently encoded by msrA/B (95.6%) gene, LSa/b phenotype by lnuA (56.3%) gene, and iMLSb phenotype by ermC (29.4%) and ermA (25.5%) genes. Although cMLSb phenotype was mostly determined by the presence of ermC (28.9%), combinations of two or more genes have been present too. This pattern was particularly recorded in methicillin-resistant Staphylococcus aureus (MRSA) (58.3%) and methicillin-resistant coagulase-negative staphylococci (MRCNS) (90.9%) isolates with cMLSB phenotype. The msrA/B gene and M/MSb phenotype were statistically significantly higher in hospital-acquired than community-acquired staphylococci strains (p < 0.05). There are no statistically significant differences between staphylococci harboring the rest of MLS resistance genes acquired in community and hospital settings (p > 0.05). The prevalence of iMLSb phenotypes may change over time, so it is necessary to perform periodic survey of MLS resistance phenotypes, particularly where the D-test is not performed routinely.
机译：大环内酯类，林可酰胺类和链霉菌素（MLS）耐药基因负责对葡萄球菌感染的这些抗生素产生耐药性。该研究的目的是分析社区和医院获得的葡萄球菌分离株中MLS抗性基因的分布。 MLS耐药表型[对大环内酯-林可酰胺-链霉菌素B（cMLSb）的诱导耐药性，对大环内酯-林可酰胺-链霉菌素B的诱导耐药性，对大环内酯/大环内酯-链霉菌素B（M / MSb）的耐药性以及对林可酰胺-耐药性链霉菌素A /链霉菌素B（LSa / b）]通过双盘扩散法测定。通过端点聚合酶链反应，在1年内从微生物学中心收集的179株葡萄球菌中，通过终点聚合酶链反应确定了MLS抗性基因（ermA，ermB，ermC，msrA / B，lnuA，lnuB和lsaA）的存在弗拉涅公共卫生研究所。在葡萄球菌中，无论是社区获得性还是医院获得性，最常见的MLS表型均为iMLSb（33.4％）。倒数第二高的是M / MSb（17.6％），在医院获得的葡萄球菌分离株数量在统计学上显着更高（p <0.05）。 MLS耐药性主要由msrA / B（35.0％）和ermC（20.8％）基因的存在决定。检查的表型主要由一个基因的存在决定，尤其是通过msrA / B（26.3％）和ermC（14.5％）来确定，而15.6％是通过两个或多个基因的组合来确定的。 M / MSb表型由msrA / B（95.6％）基因编码，LSa / b表型由lnuA（56.3％）基因编码，而iMLSb表型由ermC（29.4％）和ermA（25.5％）基因编码。尽管cMLSb表型主要由ermC（28.9％）的存在决定，但也存在两个或多个基因的组合。这种模式尤其记录在具有cMLSB表型的耐甲氧西林金黄色葡萄球菌（MRSA）（58.3％）和耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）（90.9％）中。在医院获得的msrA / B基因和M / MSb表型在统计学上显着高于社区获得的葡萄球菌菌株（p <0.05）。具有社区和医院环境中获得的其余MLS抗性基因的葡萄球菌之间无统计学差异（p> 0.05）。 iMLSb表型的患病率可能随时间变化，因此有必要对MLS耐药表型进行定期调查，尤其是在不定期进行D检验的情况下。

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