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Diversity and effect of Trichoderma spp. associated with green mold disease on Lentinula edodes in China

机译:木霉属物种的多样性和作用。与香菇绿色霉菌病相关

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Abstract Lentinula edodes , one of the most important edible mushrooms in China, is affected heavily by the infection of green mold that overgrows mushroom mycelia. We collected the diseased samples from main L. edodes cultivation regions in China to characterize the pathogen and to study the effect of Trichoderma spp. on L. edodes species. We identified six Trichoderma species, that is, T. harzianum , T. atroviride , T. viride , T. pleuroticola , T. longibrachiatum, and T. oblongisporum based on the internal transcribed spacer or tef1- ???± sequences and morphology characteristics. In confrontation cultures on Petri plates or in tubes, and in L. edodes cultures in a medium containing Trichoderma metabolites, L. edodes mycelia were not only distorted and swollen, but also inhibited by Trichoderma isolates. It is not possible that adjusting pH value or temperature is used for controlling L. edodes green disease, because the growth of most of Trichoderma isolates and L. edodes shared similar pH and temperature conditions.
机译:摘要香菇是中国最重要的食用菌之一,它受到过度生长的蘑菇菌丝体绿色霉菌的感染。我们从中国主要的香菇栽培区收集了患病标本,以鉴定病原菌并研究木霉菌的效果。在L. edodes种类上。我们根据内部转录的间隔区或tef1-Δ±序列和形态特征,鉴定了6种木霉菌种,即哈茨木霉,阿曲韦雷德,阿维韦德木霉,特里弗德木霉,胸膜肺炎衣原体,长曲霉和长圆孢子菌。 。在培养皿或试管中的对抗培养物中,以及在含有木霉属代谢产物的培养基中的香菇培养物中,香菇菌丝体不仅被扭曲和溶胀,而且还被木霉分离物抑制。不可能使用调节pH值或温度来控制香菇绿色疾病,因为大多数木霉分离物和香菇的生长具有相似的pH和温度条件。

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