...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Memorias do Instituto Oswaldo Cruz >Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors
【24h】

Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors

机译：重组丙型肝炎病毒包膜蛋白2与低密度脂蛋白/ CD81受体的相互作用

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hepatitis C virus (HCV) envelope protein 2 (E2) is involved in viral binding to host cells. The aim of this work wasto produce recombinant E2B and E2Y HCV proteins in Escherichia coli and Pichia pastoris, respectively, and to studytheir interactions with low-density lipoprotein receptor (LDLr) and CD81 in human umbilical vein endothelial cells(HUVEC) and the ECV304 bladder carcinoma cell line. To investigate the effects of human LDL and differences inprotein structure (glycosylated or not) on binding efficiency, the recombinant proteins were either associated or notassociated with lipoproteins before being assayed. The immunoreactivity of the recombinant proteins was analysedusing pooled serum samples that were either positive or negative for hepatitis C. The cells were immunophenotypedby LDLr and CD81 using flow cytometry. Binding and binding inhibition assays were performed in the presence ofLDL, foetal bovine serum (FCS) and specific antibodies. The results revealed that binding was reduced in the absenceof FCS, but that the addition of human LDL rescued and increased binding capacity. In HUVEC cells, the useof antibodies to block LDLr led to a significant reduction in the binding of E2B and E2Y. CD81 antibodies did notaffect E2B and E2Y binding. In ECV304 cells, blocking LDLr and CD81 produced similar effects, but they were not asmarked as those that were observed in HUVEC cells. In conclusion, recombinant HCV E2 is dependent on LDL forits ability to bind to LDLr in HUVEC and ECV304 cells. These findings are relevant because E2 acts to anchor HCVto host cells; therefore, high blood levels of LDL could enhance viral infectivity in chronic hepatitis C patients.
机译：丙型肝炎病毒（HCV）包膜蛋白2（E2）参与病毒与宿主细胞的结合。这项工作的目的是分别在大肠杆菌和巴斯德毕赤酵母中生产重组E2B和E2Y HCV蛋白，并研究它们与人脐静脉内皮细胞（HUVEC）和ECV304膀胱中的低密度脂蛋白受体（LDLr）和CD81的相互作用癌细胞系。为了研究人LDL和蛋白质结构差异（糖基化与否）对结合效率的影响，在检测重组蛋白之前，将其与脂蛋白结合或不结合。使用合并的丙型肝炎病毒阳性或阴性血清样本分析重组蛋白的免疫反应性。使用流式细胞术通过LDLr和CD81对细胞进行免疫表型分析。在LDL，胎牛血清（FCS）和特异性抗体的存在下进行结合和结合抑制测定。结果表明，在不存在FCS的情况下，结合减少了，但是人LDL的加入挽救了结合能力，并增加了结合能力。在HUVEC细胞中，使用抗体阻断LDLr导致E2B和E2Y的结合显着降低。 CD81抗体不影响E2B和E2Y结合。在ECV304细胞中，阻断LDLr和CD81产生了相似的作用，但没有像在HUVEC细胞中观察到的那样被标记。总之，重组HCV E2依赖LDL结合HUVEC和ECV304细胞中LDLr的能力。这些发现是相关的，因为E2可将HCV锚定在宿主细胞上。因此，高浓度的低密度脂蛋白可以增强慢性丙型肝炎患者的病毒感染性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号