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首页> 外文期刊>Nucleus >UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity
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UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity

机译：UBF与磷脂酰肌醇4,5-二磷酸在核仁组织区中的复合物，无论正在进行的RNA聚合酶I活性如何

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摘要

To maintain growth and division, cells require a large-scale production of rRNAs which occurs in the nucleolus. Recently, we have shown the interaction of nucleolar phosphatidylinositol 4,5-bisphosphate (PIP2) with proteins involved in rRNA transcription and processing, namely RNA polymerase I (Pol I), UBF, and fibrillarin. Here we extend the study by investigating transcription-related localization of PIP2 in regards to transcription and processing complexes of Pol I. To achieve this, we used either physiological inhibition of transcription during mitosis or inhibition by treatment the cells with actinomycin D (AMD) or 5,6-dichloro-1β-d-ribofuranosyl-benzimidazole (DRB). We show that PIP2 is associated with Pol I subunits and UBF in a transcription-independent manner. On the other hand, PIP2/fibrillarin colocalization is dependent on the production of rRNA. These results indicate that PIP2 is required not only during rRNA production and biogenesis, as we have shown before, but also plays a structural role as an anchor for the Pol I pre-initiation complex during the cell cycle. We suggest that throughout mitosis, PIP2 together with UBF is involved in forming and maintaining the core platform of the rDNA helix structure. Thus we introduce PIP2 as a novel component of the NOR complex, which is further engaged in the renewed rRNA synthesis upon exit from mitosis.
机译：为了维持生长和分裂，细胞需要大规模生产核仁中存在的rRNA。最近，我们已经显示了核仁磷脂酰肌醇4,5-二磷酸（PIP2）与参与rRNA转录和加工的蛋白质，即RNA聚合酶I（Pol I），UBF和原纤维蛋白的相互作用。在这里，我们通过研究与Pol I转录和加工复合物有关的PI​​P2的转录相关定位来扩展研究。为实现这一目标，我们在有丝分裂期间使用了生理学上的转录抑制作用，或者通过用放线菌素D（AMD）或5,6-二氯-1β-d-呋喃呋喃糖基-苯并咪唑（DRB）。我们显示PIP2以转录独立的方式与Pol I亚基和UBF相关联。另一方面，PIP2 /原纤维蛋白共定位取决于rRNA的产生。这些结果表明，PIP2不仅在rRNA产生和生物发生期间是必需的（如我们先前所示），而且在细胞周期中作为Pol I预起始复合物的锚也起结构性作用。我们建议在整个有丝分裂期间，PIP2与UBF一起参与形成和维持rDNA螺旋结构的核心平台。因此，我们将PIP2作为NOR复合物的新成分引入，该复合物在有丝分裂退出后进一步参与了更新的rRNA合成。

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