Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription

Sobol Margarita; Krausova Alzbeta; Yildirim Sukriye; Kalasova Ilona; Faberova Veronika; Vrkoslav Vladimir; Philimonenko Viada; Marasek Pavel; Pastorek Lukas; Capek Martin; Lubovska Zuzana; Ulicna Livia; Tsuji Takuma; Lisa Miroslav; Cvacka Josef; Fujimoto Toyoshi; Hozak Pavel

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Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription

机译：核磷酸酰肌醇4,5-双磷酸胰岛有助于有效的RNA聚合酶II依赖性转录

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This paper describes a novel type of nuclear structure - nuclear lipid islets (NLIs). They are of 40-100 nm with a lipidic interior, and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] molecules comprise a significant part of their surface. Most of NLIs have RNA at the periphery. Consistent with that, RNA is required for their integrity. The NLI periphery is associated with Pol II transcription machinery, including the largest Pol II subunit, transcription factors and NM1 (also known as NMI). The Ptdlns(4,5)P-2-NM1 interaction is important for Pol II transcription, since NM1 knockdown reduces the Pol II transcription level, and the overexpression of wild-type NM1 [but not NM1 mutated in the Ptdlns(4,5)P-2-binding site] rescues the transcription. Importantly, Pol II transcription is dependent on NLI integrity, because an enzymatic reduction of the Ptdlns(4,5)P-2 level results in a decrease of the Pol II transcription level. Furthermore, about half of nascent transcripts localise to NLIs, and transcriptionally active transgene loci preferentially colocalise with NLIs. We hypothesize that NLIs serve as a structural platform that facilitates the formation of Pol II transcription factories, thus participating in the formation of nuclear architecture competent for transcription.

机译：本文描述了一种新型核结构 - 核脂胰岛（NLI）。它们具有40-100nm，具有脂质内部，磷脂酰肌醇4,5-双磷酸溶胶[ptdins（4,5）p-2]分子包含其表面的重要部分。大多数Nlis都有周边的RNA。与此符合，RNA是完整性的。 Nli周边与Pol II转录机构相关，包括最大的Pol II亚基，转录因子和NM1（也称为NMI）。 PTDLNS（4,5）P-2-NM1相互作用对于POL II转录很重要，因为NM1敲低减少了POL II转录水平，并且野生型NM1的过度表达[但不是在PTDLNS中突变的NM1（4,5 ）p-2结合位点]拯救转录。重要的是，POL II转录取决于Nli完整性，因为PTDLNS（4,5）P-2水平的酶促减少导致POL II转录水平的降低。此外，大约一半的新生转录物定位为NLI，并且通过NLIS优先分层的转录活性转基因座。我们假设NLIS作为结构平台，促进了POL II转录工厂的形成，从而参与形成核建筑的职能转录。

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《Journal of Cell Science》 |2018年第8期|共17页
作者
Sobol Margarita; Krausova Alzbeta; Yildirim Sukriye; Kalasova Ilona; Faberova Veronika; Vrkoslav Vladimir; Philimonenko Viada; Marasek Pavel; Pastorek Lukas; Capek Martin; Lubovska Zuzana; Ulicna Livia; Tsuji Takuma; Lisa Miroslav; Cvacka Josef; Fujimoto Toyoshi; Hozak Pavel;
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作者单位

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Res Serv Grp Mass Spectrometry Vvi Inst Organ Chem &

Biochem Flemingovo Namesti 2 Prague;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Light Microscopy Core Facil Videnska 1083 Prague 14220 4 Czech Republic;

CAS Vvi Inst Mol Genet Electron Microscopy Core Facil Videnska 1083 Prague 14220 4 Czech;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

Nagoya Univ Grad Sch Med Dept Mol Cell Biol Showa Ku 65 Tsurumai Cho Nagoya Aichi 4668550;

Univ Pardubice Fac Chem Technol Dept Analyt Chem Studentska 573 Pardubice 53210 Czech Republic;

CAS Res Serv Grp Mass Spectrometry Vvi Inst Organ Chem &

Biochem Flemingovo Namesti 2 Prague;

Nagoya Univ Grad Sch Med Dept Mol Cell Biol Showa Ku 65 Tsurumai Cho Nagoya Aichi 4668550;

CAS Vvi Inst Mol Genet Dept Biol Cell Nucleus Videnska 1083 Prague 14220 4 Czech Republic;

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正文语种 eng
中图分类细胞生物学;
关键词
Nucleus; PtdIns(4; 5)P-2; DNA transcription; Chromatin; RNA polymerase II; Phosphoinositides;

机译：核;PTDINS（4;5）p-2;DNA转录;染色质;RNA聚合酶II;磷酸钠;

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专利

1. Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription [J] . Sobol Margarita, Krausova Alzbeta, Yildirim Sukriye, Journal of Cell Science . 2018,第8期

机译：核磷酸酰肌醇4,5-双磷酸胰岛有助于有效的RNA聚合酶II依赖性转录
2. Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription [J] . YildirimS., CastanoE., SobolM., Journal of Cell Science . 2013,第12期

机译：磷脂酰肌醇4,5-二磷酸参与RNA聚合酶I转录
3. The Direct Interaction Between ASH2, a Drosophila Trithorax Group Protein, and SKTL, a Nuclear Phosphatidylinositol 4-Phosphate 5-Kinase, Implies a Role for Phosphatidylinositol 4,5-Bisphosphate in Maintaining Transcriptionally Active Chromatin [J] . Allen Shearn, Mimi K. Cheng Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2004,第3期

机译：果蝇Trithorax组蛋白ASH2与核磷酸磷脂酰肌醇4磷酸5激酶SKTL之间的直接相互作用暗示了磷脂酰肌醇4,5-双磷酸酯在维持转录活性染色质中的作用。
4. Atypical Transcription Factors Function with RNA Polymerase II in Trypanosomes to Produce the Spliced Leader RNA [C] . V. Bellofatto, J.B. Palenchar, A. Da International Congress of Parasitology . 2006

机译：非典型转录因子在锥虫中具有RNA聚合酶II的功能，以产生剪接前导RNA
5. Live Cell Super-Resolution Microscopy Quanitifies an Interaction Between Influenza Hemagglutinin and Phosphatidylinositol 4,5-Bisphosphate [D] . Wallace, Jaqulin Nicole. 2020

机译：Live Cell超分辨率显微镜刺激流感血凝素和磷脂酰肌醇4,5-双磷酸酯之间的相互作用
6. CHD8 Associates with Human Staf and Contributes to Efficient U6 RNA Polymerase III Transcription [O] . Chih-Chi Yuan, Xinyang Zhao, Laurence Florens, 2007

机译：CHD8与人类工作人员相关联并为有效的U6 RNA聚合酶III转录做出贡献
7. The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin. [O] . Cheng, Mimi K, Shearn, Allen 2004

机译：果蝇三胸草蛋白ASH2与核磷脂酰肌醇4磷酸5激酶SKTL之间的直接相互作用，暗示磷脂酰肌醇4，5-二磷酸在维持转录活性染色质中的作用。

Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription

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