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首页> 外文期刊>Regenerative Therapy >Asperosaponin VI stimulates osteogenic differentiation of rat adipose-derived stem cells
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Asperosaponin VI stimulates osteogenic differentiation of rat adipose-derived stem cells

机译：过氧化物酶VI刺激大鼠脂肪干细胞的成骨分化

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In the aging population, the decrease on osteogenic differentiation resulted into a significant reduction in bone formation. Bone tissue engineering has been a successful technique for treatment of bone defects. It is reported that adipose-derived stem cells (ADSCs) have pluripotency to differentiate into adipocytes and osteoblasts. However little is revealed about the effect of the herbal medicine Asperosaponin VI (ASA VI) on ADSCs differentiation. In our study, we isolated and identified ADSCs from rats. We examined the effect of different concentrations of ASA VI in ADSCs on alkaline phosphatase (ALP) activity, calcium deposition, the expression of bone-related proteins and the release of inflammatory cytokines. Flowcytometry assay showed ADSCs were highly expressed CD44 and CD105, but hardly expressed CD34 and CD45, suggesting ADSCs were successfully isolated for follow-up experiments. ALP activity examination and Alizarin red (AR) stain showed that ASA VI enhanced the ALP activity and promoted matrix mineralization in ADSCs. In addition, bone-related protein OCN and RUNX2, and Smad2/3 phosphorylation was upregulated after ASA VI treatment in ADSCs. ELISA results showed that ASA VI blocked the release of TNF-α, IL-6 and IL-1β in ADSCs. Considering this results, we concluded that ASA VI promotes osteogenic differentiation of ADSCs through inducing the expression of bone-related proteins. These findings enriched the function of ASA VI as a regenerative medicine and shed new light for the treatment of bone defects in clinical research.
机译：在老龄化人群中，成骨分化的减少导致骨形成的显着减少。骨组织工程学已经成为治疗骨缺损的成功技术。据报道，脂肪干细胞（ADSCs）具有多能分化为脂肪细胞和成骨细胞的能力。然而，关于中草药Asperosaponin VI（ASA VI）对ADSCs分化的影响却鲜有揭示。在我们的研究中，我们从大鼠中分离并鉴定了ADSC。我们检查了ADSC中不同浓度的ASA VI对碱性磷酸酶（ALP）活性，钙沉积，骨相关蛋白的表达和炎性细胞因子释放的影响。流式细胞仪检测显示ADSCs高表达CD44和CD105，但几乎不表达CD34和CD45，表明ADSCs已成功分离用于后续实验。 ALP活性检查和茜素红（AR）染色表明，ASA VI增强了ADSC中的ALP活性并促进了基质矿化。此外，在ADSC中进行ASA VI治疗后，与骨相关的蛋白OCN和RUNX2以及Smad2 / 3磷酸化被上调。 ELISA结果表明，ASA VI阻断了ADSCs中TNF-α，IL-6和IL-1β的释放。考虑到这一结果，我们得出结论，ASA VI通过诱导骨相关蛋白的表达来促进ADSC的成骨分化。这些发现丰富了ASA VI作为再生药物的功能，为临床研究骨缺损的治疗提供了新的思路。

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