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首页> 外文期刊>Revista de salud animal >DETECTION BY PCR Anaplasma marginale IN BUFFALOES OF THE WESTERN REGION OF CUBA
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DETECTION BY PCR Anaplasma marginale IN BUFFALOES OF THE WESTERN REGION OF CUBA

机译：古巴西部地区水牛的PCR无性生殖检测

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The buffalo species was recently introduced in Cuba, showing excellent productive and reproductive performance. Their health situation has been little studied, although their natural resistance to various diseases reported in the country, including haemoparasitosis has been recognized. Buffaloes are also susceptible to infection with Anaplasma marginale, but hardly present clinical anaplasmosis, remaining asymptomatic carriers, which facilitates transmission to cattle. This study is aimed at determining the presence of A. marginale in buffaloes of the western region of Cuba. For this purpose, an nPCR test, based on msp5 gene amplification of this hemoparasites, was developep. Fifty-two DNA samples, extracted from animal blood, randomly selected from 6 farms in western Cuba, which underwent blood smear were analized. From the total samples analyzed, 47 (90.4%) were positive by nPCR, visualizing in all cases a band of 345 bp, while only 5 animals (9.6%) were positive in blood smear. One of the fragment amplified was sequenced and the sequence obtained was deposited in GenBank under Accession Number JF270381, showing a high percentage of identity with Anaplasma marginale deposited sequences in different regions of the world.
机译：水牛物种最近被引入古巴，表现出极好的生产和繁殖性能。尽管已经认识到他们对本国报道的对各种疾病包括血寄生虫病的天然抵抗力，但对其健康状况的研究很少。水牛也容易被边缘无形体感染，但几乎没有临床无形体病，残留无症状的携带者，这有利于牛的传播。这项研究的目的是确定古巴西部水牛中A. marginale的存在。为此目的，开发了基于该血寄生虫的msp5基因扩增的nPCR测试。对动物血液中提取的52个DNA样本进行了分析，这些样本是从古巴西部6个农场中随机抽取的动物血液中提取的。在分析的全部样本中，通过nPCR显示47例（90.4％）呈阳性，在所有情况下均显示345 bp的条带，而只有5只动物（9.6％）的血液涂片呈阳性。对扩增的片段之一进行测序，并将获得的序列以登录号JF270381保藏在GenBank中，显示出与世界不同地区的无缘无浆膜保藏序列的同一性高。

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