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WAVELENGTH DEPENDENCE OF THE ELECTRIC SIGNALS GENERATED IN DRIED BR SAMPLES

机译:干燥BR样品中产生的电信号的波长依赖性

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The electrical signals from Bacteriorhodopsin-containing air dried purple membranes (PM) were recorded at various wavelengths by using a 250 W halogen lamp and narrow band interference filters to excite the probe. The amplitude of the signals was plotted as a function of the wavelength and then compared to the absorption spectrum of dried PMs. The action spectrum thus obtained shows a good correlation with the absorption spectrum in the long wavelengths region but not in the short wavelengths one in contrast to the results obtained in AFM measurements where the correlation was very good at all wavelengths. This puzzling result was tentatively explained taking into account the fact that the samples were excited with continuous light having as a consequence the simultaneous presence of several intermediates of the photocycle, each with a different electrogenicity. Further experiments, employing as exciting laser pulses of various wavelengths, are required in order to elucidate this problem.
机译:通过使用250 W卤素灯和窄带干涉滤光片激发探针,以各种波长记录了含细菌视紫红质的空气干燥紫色膜(PM)的电信号。将信号的幅度绘制为波长的函数,然后与干燥的PM的吸收光谱进行比较。如此获得的作用谱与长波长区域中的吸收光谱具有良好的相关性,而与短波长区域中的吸收光谱没有良好的相关性,这与在所有波长下的相关性都非常好的AFM测量中获得的结果相反。考虑到样品被连续光激发的事实,暂时解释了这种令人困惑的结果,结果是连续存在光循环的几种中间体,每种中间体具有不同的电原性。为了阐明这个问题,需要进一步的实验,采用各种波长的激发激光脉冲。

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