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首页> 外文期刊>Romanian Journal of Biophysics >LOW LEVEL LONG WAVELENGTH LASER IRRADIATION EFFECTS ON HUMAN T LEUKEMIC LYMPHOBLASTS MITOCHONDRIAL MEMBRANE POTENTIAL
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LOW LEVEL LONG WAVELENGTH LASER IRRADIATION EFFECTS ON HUMAN T LEUKEMIC LYMPHOBLASTS MITOCHONDRIAL MEMBRANE POTENTIAL

机译：低水平长波长激光辐照对人T淋巴细胞淋巴母细胞线粒体膜电位的影响

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摘要

We investigated the effects of low power 680 nm far-red and 830 nm near-infrared laser light on noninjured and energyutrient restricted human acute T leukemic Jurkat cells mitochondrial membrane state. Nutrient restriction engendered increase in mitochondrial membrane potential (??m) of the cell subpopulation with highly polarized mitochondrial membrane (HIGH), and decrease in ??m of the subpopulation with low polarization mitochondrial membrane (LOW), changes induced by glucose deprivation with blockade of glycolysis being more substantial than those brought on by serum starvation. Glucose starvation also caused increase in the relative magnitude of the HIGH subpopulation, while serum starvation resulted in decrease in the relative magnitude of the HIGH subpopulation. Energy restriction induced by mild blockade of oxidative phosphorylation with low concentrations of cyanide caused increase in the relative magnitude of the HIGH subpopulation, while high concentration cyanide poisoning promoted mitochondrial membrane depolarization. Low level near infrared laser irradiation caused decrease in the HIGH subpopulation in control cell populations, partially reversed glucose starvation caused effects, while had no significant influence on mitochondrial membrane state in serum starved cells. Low level far-red laser irradiation induced increase in the relative magnitude of the HIGH subpopulation, and partially reversed mild and severe cyanide intoxication effects. Intermediate level cyanide intoxication caused increase in the relative magnitude of the HIGH subpopulation could also be enhanced by far-red laser radiation. In conclusion, our data show that soft laser irradiation significantly modulates the control and nutrient/energy restricted human T leukemia lymphoblasts mitochondrial membrane state in a dose and irradiation regime dependent manner.
机译：我们研究了低功率680 nm远红外线和830 nm近红外激光对无伤害和受能量/营养限制的人类急性T白血病Jurkat细胞线粒体膜状态的影响。营养限制导致高极化线粒体膜（HIGH）的细胞亚群的线粒体膜电位（Δεm）增加，而低极化线粒体膜（LOW）的亚群的Δεm减少，这是葡萄糖剥夺引起的。糖酵解的阻断作用比血清饥饿引起的阻断作用更为重要。葡萄糖饥饿也引起HIGH亚群的相对量的增加，而血清饥饿导致HIGH亚群的相对量的减少。低浓度氰化物对氧化磷酸化的轻度阻断引起的能量限制导致HIGH亚群的相对量增加，而高浓度氰化物中毒则促进了线粒体膜的去极化。低水平的近红外激光辐照导致对照细胞群中HIGH亚群的减少，部分逆转了葡萄糖饥饿引起的效应，而对血清饥饿细胞中的线粒体膜状态没有显着影响。低水平的远红外激光辐照引起HIGH亚群的相对量增加，并部分逆转了轻度和重度氰化物中毒作用。远红外激光辐射也可以增强中等水平的氰化物中毒，引起HIGH亚群相对数量的增加。总之，我们的数据表明，软激光辐照以剂量和辐照方式依赖性方式显着调节对照和受营养/能量限制的人类T白血病淋巴母细胞线粒体膜状态。

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