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首页> 外文期刊>Romanian Journal of Biophysics >INTERACTION BETWEEN CEFTAZIDIME AND BACTERIAL PORIN OMPF ANALYZED BY FLUORESCENCE
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INTERACTION BETWEEN CEFTAZIDIME AND BACTERIAL PORIN OMPF ANALYZED BY FLUORESCENCE

机译：荧光分析头孢他啶与细菌性孔蛋白OMPF之间的相互作用

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摘要

Porins found in the outer membrane of Gram negative bacteria are considered to be one of the pathways used by antibiotics to cross the membrane. However, in time, bacteria have developed different mechanisms to fight the antibiotics and preventing them to reach the target. Studying the interaction between outer membrane porin F (OmpF) and antibiotics will help understand better these mechanisms. The insertion the OmpF (extracted form Escherichia coli) into homogeneous and heterogeneous liposomes membranes was studied by fluorescence resonance energy transfer (FRET) technique. The quenching of OmpF tryptophane residues fluorescence by ceftazidime (a ?-lactam family antibiotic) allowed describing the interaction between the antibiotic and the porin.
机译：在革兰氏阴性细菌外膜中发现的孔蛋白被认为是抗生素穿过膜的途径之一。但是，随着时间的流逝，细菌已经开发出不同的机制来对抗抗生素并阻止它们到达目标。研究外膜孔蛋白F（OmpF）与抗生素之间的相互作用将有助于更好地了解这些机制。通过荧光共振能量转移（FRET）技术研究了将OmpF（从大肠杆菌中提取）插入均质和异质脂质体膜中的过程。头孢他啶（α-内酰胺类抗生素）对OmpF色氨酸残基的荧光猝灭可以描述抗生素和孔蛋白之间的相互作用。

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