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首页> 外文期刊>Physiological Reports >Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha
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Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

机译：牛子宫内膜细胞炎性介质表达的调节：脂多糖，白介素1β和肿瘤坏死因子α的影响。

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摘要

An abnormal uterine environment can influence maternal–fetal communication, conception rate and disrupt normal embryo development, thereby affecting fertility and the reproductive performance of dairy cows. Animal variability means that development of endometrial cell lines with appropriate characteristic are required. We evaluated the effect of an infectious agent (i.e., bacterial lipopolysaccharide; LPS) and proinflammatory mediators (i.e., Interleukin 1 beta; IL‐1 β , and tumor necrosis factor alpha; TNF α ) on inflammatory mediator gene expression and production by bovine endometrial epithelial (bEEL) and stromal (bCSC) cell lines. Expression of CXCL8/IL8, IL1A, IL1B , and IL6 cytokine genes was significantly upregulated in both epithelial and stromal cells when treated with LPS and IL‐1 β . LPS treatment of epithelial cells (compared with treatment by IL‐1 β and TNF α ) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Whereas, in stromal cells, IL‐1 β treatment (compared with LPS and TNF α ) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Interestingly, bEEL and bCSC cells treated with IL‐1 β increased IL1B gene expression, suggesting that IL‐1 β may act unusually in an autocrine‐positive feedback loop. Cytokine production was stimulated by these agents in both cell types. We suggest that the characteristics of these two cell lines make them excellent tools for the study of intrauterine environment.
机译：异常的子宫环境会影响母婴沟通，受孕率并破坏正常的胚胎发育，从而影响奶牛的生育能力和生殖性能。动物变异性意味着需要发展具有适当特征的子宫内膜细胞系。我们评估了感染因子（即细菌脂多糖; LPS）和促炎介质（即白介素1 beta; IL-1β和肿瘤坏死因子α; TNFα）对炎性介质基因表达和牛子宫内膜产生的影响。上皮（bEEL）和基质（bCSC）细胞系。用LPS和IL-1β处理后，上皮和基质细胞中CXCL8 / IL8，IL1A，IL1B和IL6细胞因子基因的表达均显着上调。 LPS处理上皮细胞（与IL-1β和TNFα相比）显示出更高的CXCL8 / IL8，IL1A，IL1B和IL6细胞因子基因表达。而在基质细胞中，IL-1β处理（与LPS和TNFα相比）表现出更高的CXCL8 / IL8，IL1A，IL1B和IL6细胞因子基因表达。有趣的是，用IL-1β处理的bEEL和bCSC细胞增加了IL1B基因的表达，表明IL-1β在自分泌阳性反馈回路中可能起异常作用。在两种细胞类型中，这些因子刺激细胞因子的产生。我们建议这两种细胞系的特性使其成为研究子宫内环境的极佳工具。

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