• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>PLOS Neglected Tropical Diseases >Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections
【24h】

Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections

机译：检测丝虫感染中循环寄生虫衍生的microRNA。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Filarial nematodes cause chronic and profoundly debilitating diseases in both humans and animals. Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investigated the presence of circulating miRNAs released by filarial nematodes into the host bloodstream. miRNA deep-sequencing combined with bioinformatics revealed over 200 mature miRNA sequences of potential nematode origin in Dirofilaria immitis-infected dog plasma in two independent analyses, and 21 in Onchocerca volvulus-infected human serum. Total RNA obtained from D. immitis-infected dog plasma was subjected to stem-loop RT-qPCR assays targeting two detected miRNA candidates, miR-71 and miR-34. Additionally, Brugia pahangi-infected dog samples were included in the analysis, as these miRNAs were previously detected in extracts prepared from this species. The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream.
机译：丝虫线虫在人和动物中引起慢性和严重衰弱的疾病。新技术的应用为改善诊断和我们对宿主-寄生虫相互作用的分子基础的理解提供了前所未有的机会。第一步，我们研究了由丝虫线虫释放到宿主血流中的循环miRNA的存在。 miRNA深度测序与生物信息学相结合，在两次独立的分析中，在Dirofilaria炎性病毒感染的狗血浆中发现了200多个潜在线虫起源的成熟miRNA序列，在圆盘螺虫感染的人血清中显示了21条成熟的miRNA序列。将从感染D.Immitis的狗血浆中获得的总RNA进行针对两个检测到的miRNA候选物miR-71和miR-34的茎环RT-qPCR分析。另外，分析中还包括感染了帕吉亚（Brugia pahangi）的狗样品，因为这些miRNA先前是在从该物种制备的提取物中检测到的。 miR-71和miR-34的存在区分了未感染样品（未发生特异性miRNA扩增）的感染样品（均为物种）。但是，两种寄生虫的绝对miRNA拷贝数与微丝虫病均无显着相关性。这可能是由于估计感染强度的mf计数不精确或由于存在的未知数量的成虫引起的miRNA贡献。尽管如此，即使对于那些不活在血液中的物种，在血浆或血清中也发现了寄生虫衍生的循环miRNA。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号