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Comparative study of biocorrective protein-peptide agent to improve quality and safety of livestock products

机译：生物校正蛋白肽试剂改善畜产品质量和安全性的比较研究

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Water with modified isotopic composition (D/H = 40 ppm) as a solubilizing agent for biologically active substances extraction from immune organs Sus Scrofa increases protein yield in thymus to 20%, in spleen up to 38%, mesenteric lymph nodes up to 35% in comparison with distilled water (D/H = 150 ppm). It was found a significant amount of neurotransmitter amino acids, such as aspartic and glutamic acids, glycine (thymus - 10.5; 13.7; 7.6%; spleen - 12.2; 10.7; 7.8% lymph nodes - 11.0; 13.3; 11.1%, respectively) having the immunological and adaptogenic activity, in extracts of Sus scrofa immunocompetent organs (thymus, spleen, mesenteric lymph nodes). Application of water with modified isotopic composition as solubilizing agent (D / H = 40 ppm) for extraction of immunocompetent organs' biologically active compounds led to increased amino acid content, including hydrophilic amino acids, in thymus extracts - up to 22%, in spleen extracts - up to 15% , in lymph nodes exctracts - up to 8%, in comparison to distilled water (D / H = 150 ppm). Peptide profile analysis revealed positive effect of water (D/H = 40 ppm) on a quality protein content extracts in molecular weight range from 10 to 20 kDa and from 30 to 45 kDa and peptide composition (2 kDa), at the same time quantitative content of compounds adaptogenic and immunocorrective action increased. Reducing of deuterium content in the solubilizing agent enhances quantitative amino acid content, i.e. extraction in an aqueous- salt solution based on deuterium depleted water of animal tissues with a high content of amino acids with hydrophilic radicals proceeded more completely.
机译：具有修饰的同位素组成（D / H = 40 ppm）的水作为增溶剂，可从免疫器官中提取生物活性物质Sus Scrofa可将胸腺中的蛋白质产量提高到20％，脾脏中的蛋白质产量增加到38％，肠系膜淋巴结的增加到35％与蒸馏水（D / H = 150 ppm）相比。发现大量的神经递质氨基酸，例如天冬氨酸和谷氨酸，甘氨酸（胸腺-10.5; 13.7; 7.6％;脾-12.2; 10.7; 7.8％淋巴结-11.0; 13.3; 11.1％）具有Sus scrofa具有免疫能力的器官（胸腺，脾脏，肠系膜淋巴结）提取物中的免疫和适应原活性。使用具有改良同位素组成的水作为增溶剂（D / H = 40 ppm）来提取免疫活性器官的生物活性化合物会导致胸腺提取物中的氨基酸含量（包括亲水性氨基酸）增加-高达22％的脾脏与蒸馏水（D / H = 150 ppm）相比，提取物-最多15％在淋巴结中可提取-最多8％。肽谱分析表明，水（D / H = 40 ppm）对分子量在10至20 kDa和30至45 kDa的高质量蛋白质提取物和肽组成（2 kDa）具有积极作用，同时定量化合物的适应原性和免疫纠正作用的含量增加。降低增溶剂中的氘含量可提高氨基酸的定量含量，即在基于氘的贫水的盐水溶液中提取动物组织，该组织中氨基酸含量高且带有亲水性基团的过程更加完全。

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    《Potravinarstvo》 |2017年第1期|共5页
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