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首页> 外文期刊>Ukrainian Biochemical Journal >Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues
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Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues

机译：腺苷硫胺素三磷酸和腺苷硫胺素三磷酸水解酶在动物组织中的活性

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Adenosine thiamine triphosphate (AThTP), a vitamin Bsub1/sub containing nucleotide with unknown biochemical role, was found previously to be present in various biological objects including bacteria, yeast, some human, rat and mouse tissues, as well as plant roots. In this study we quantify AThTP in mouse, rat, bovine and chicks. We also show that in animal tissues the hydrolysis of AThTP is catalyzed by a membrane-bound enzyme seemingly of microsomal origin as established for rat liver, which exhibits an alkaline pH optimum of 8.0-8.5 and requires no Mgsup2+/sup ions for activity. In liver homogenates, AThTP hydrolase obeys Michaelis-Menten kinetics with apparent K subm/sub values of 84.4 ± 9.4 and 54.6 ± 13.1 μМ as estimated from the Hanes plots for rat and chicken enzymes, respectively. The hydrolysis of AThTP has been found to occur in all samples examined from rat, chicken and bovine tissues, with liver and kidney being the most abundant in enzyme activity. In rat liver, the activity of AThTP hydrolase depends on the age of animals.
机译：以前发现含有生化作用未知的核苷酸B 1 的维生素B 1 腺苷硫胺素三磷酸（AThTP）存在于包括细菌，酵母，某些人，大鼠和小鼠组织在内的各种生物物体中作为植物的根。在这项研究中，我们定量了小鼠，大鼠，牛和小鸡中的AThTP。我们还表明，在动物组织中，AThTP的水解作用是由一种膜结合的酶催化的，该酶似乎是为大鼠肝脏建立的微粒体来源的，其碱性pH最佳为8.0-8.5，不需要Mg 2 + m 的K m 值分别根据大鼠和鸡酶的Hanes图估计为84.4±9.4和54.6±13.1μM。已发现在所有从大鼠，鸡和牛组织中检出的样品中均发生AThTP的水解，其中肝脏和肾脏的酶活性最丰富。在大鼠肝脏中，AThTP水解酶的活性取决于动物的年龄。

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