Evaluation of Diur-08 A Polyherbal Formulation for Diuretic Activity

M. Ashoka Shenoy; C.S. Shastry

摘要

In the present study diuretic activity of Diur-08, a polyherbal formulation was studied. The animals were divided into three groups of six animals each. All the animals received priming dose of 0.9% sodium chloride solution (25 ml/Kg body weight.). The first group served as control and the second group received the standard drug Spiranolactone (20 mg/Kg body weight) in 0.9% sodium chloride solution. The other group received Diur-08 at a dose of 150mg/Kg body weight suspended in 0.9% sodium chloride solution (post oral.).Urine volume was measured for all the groups for 5h. Urinary levels of sodium, potassium and chloride were estimated. Both spironolactone and formulation Diur-08 treated animals shown significant diuretic activity compared to control animals. Also there is significant increase in Na+ and Cl- excretion in treated animals when compared to control animals. We observed a potent diuretic and electrolyte excretion activity of Diur-08 formulation. Introduction Diuretics are drugs that increase the rate of urine flow, sodium excretion and are used to adjust the volume and composition of body fluids in a variety of clinical situations. Drug-induced diuresis is beneficial in many life-threatening disease conditions such as congestive heart failure, nephritic syndrome, cirrhosis, renal failure, hypertension, and pregnancy toxaemia. [1] Most diuretic drugs have the adverse effect on quality of life including impotence, fatigue, and weakness. Naturally occurring diuretics include caffeine in coffee, tea, and cola, which inhibit Na + reabsorption and alcohol in beer, wine inhibit secretion of ADH. [2], [3] Although most of the diuretics proved to be very effective in promoting sodium excretion, all cause potassium loss and prompted the search for potassium sparing diuretic. Hence search for a new diuretic agent that retains therapeutic efficacy and yet devoid of potassium loss is justified. [4]Many indigenous drugs have been claimed to have diuretic effect in Ayurvedic system.Among the several plants, Crataeva nurvala Dolichos biflorus, Tribulus terrestris, Dendrophthoe falcata, Boerhaavia diffusa, Saccharum officinarum, Butea frondosa, Boerhaavia repens, Boerhaavia rependa, Homonia riparia and Centratherum anthelmintivum have shown excellent diuretic activity. [56789101112] Diur-08 is a polyherbal formulation containing aqueous extracts of Centratherum anthelmintivum, Boerhaavia diffusa, Saccharum officinarum and Butea frondosa. The present study has been planned to evaluate diuretic activity of Diur-08 in healthy albino rats. Materials and Methods AnimalsAlbino Wistar rats weighing 175-200 g are procured from Indian Institute of Sciences. They are maintained under standard conditions (temperature 22 ± 2 oC, relative humidity 60 ± 5% and 12 h light/dark cycle).The animals were housed in sanitized polypropylene cages containing sterile paDiur-08y husk as beDiur-08ing. They had free access to standard pellet diet and water ad libitum . The Institutional Animal Ethics Committee approved the experimental protocol. All the animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” prepared by the “National Academy of Sciences” and published by the “National Institute of Health”. Drugs and ChemicalsAll the drugs, chemicals, and reagents were procured from S.D. Fine Chemicals, (Mumbai, India). All the chemicals were of analytical grade.Diuretic ActivityThe method described by Lipschtiz et al [13] and Kavimani et al [14] was employed for the assessment of diuretic activity. The animals were divided into three groups of six animals each and they were fasted and deprived of food and water for 18 h prior to experiment. All the animals received priming dose of 0.9% sodium chloride solution (25 ml/Kg body weight.). The first group received vehicle (saline) served as control and the second group served as the standard group, received the drug Spiranolactone (20 mg/Kg body wei

机译：在本研究中Diur-08的利尿活性中，研究了一种多草药配方。将动物分成三组，每组六只动物。所有动物均接受0.9％氯化钠溶液的初次剂量（25 ml / Kg体重）。第一组作为对照组，第二组接受在0.9％氯化钠溶液中的标准药物螺内酯（20 mg / Kg体重）。另一组接受以0.9％氯化钠溶液悬浮的150 mg / Kg体重的Diur-08口服（口服后），所有组均测量尿液体积5小时。估算了尿中钠，钾和氯的含量。与对照动物相比，螺内酯和Diur-08制剂处理的动物均表现出明显的利尿活性。与对照组动物相比，治疗动物的Na +和Cl-排泄量也显着增加。我们观察到Diur-08制剂具有强大的利尿和电解质排泄活性。简介利尿剂是增加尿液流动速度，钠排泄的药物，并在各种临床情况下用于调节体液的体积和成分。药物引起的利尿作用在许多危及生命的疾病中都是有益的，例如充血性心力衰竭，肾病综合征，肝硬化，肾衰竭，高血压和妊娠毒血症。 [1]大多数利尿药对生活质量有不良影响，包括阳ot，疲劳和虚弱。天然的利尿剂包括咖啡，茶和可乐中的咖啡因，它们可抑制Na +的再吸收，而啤酒中的酒精可抑制葡萄酒中ADH的分泌。 [2]，[3]尽管多数利尿剂被证明对促进钠排泄非常有效，但所有这些都会导致钾的流失，并促使人们寻找节约钾的利尿剂。因此，寻找能够保持治疗功效而又不失钾的新型利尿剂是合理的。 [4]据称许多本土药物在印度草医学系统中具有利尿作用。在几种植物中，Cretaeva nurvala dolichos biflorus，Tribulus terrestris，Dendrophthoe falcata，Boerhaavia diffusa，Saccharum officinarum，Butea frondosa，Boerhaavia repens，Boerhaavia repens，Boerhavia和Centatherumum anthelmintivum表现出优异的利尿活性。 [56789101112] Diur-08是一种多草药制剂，其中包含无烟中枢，白花蛇舌草，厚皮糖和frea frondosa的水提取物。本研究已计划评估健康白化病大鼠中Diur-08的利尿活性。材料和方法动物从印度科学院购买重175-200 g的白化Wistar大鼠。将它们保持在标准条件下（温度22±2 oC，相对湿度60±5％，明/暗循环12 h）。将动物饲养在消毒的聚丙烯笼中，该笼中装有不育的paDiur-08y外壳，如beDiur-08ing。他们可以自由享用标准的颗粒饮食和水。机构动物伦理委员会批准了实验方案。所有动物均按照“美国国家科学院”编写并由“美国国立卫生研究院”出版的“实验动物的护理和使用指南”中概述的标准接受了人文关怀。药物和化学药品所有药物，化学药品和试剂均购自S.D.精细化工（印度孟买）。所有化学药品均为分析纯。利尿活性采用Lipschtiz等人[13]和Kavimani等人[14]所述的方法评估利尿剂的活性。将动物分成三组，每组六只动物，在实验前禁食禁食18小时。所有动物均接受0.9％氯化钠溶液的初次剂量（25 ml / Kg体重）。第一组接受媒介物（盐水）作为对照组，第二组接受标准组，接受螺旋体内酯（20 mg / Kg体重

Evaluation of Diur-08 A Polyherbal Formulation for Diuretic Activity

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