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首页> 外文期刊>The Journal of Pure and Applied Chemistry Research >In Vitro and In Silico Studies of Immobilized Xylanase on Zeolite Matrix Activated with Hydrochloric Acid
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In Vitro and In Silico Studies of Immobilized Xylanase on Zeolite Matrix Activated with Hydrochloric Acid

机译：盐酸活化沸石分子筛上固定木聚糖酶的体外和计算机模拟研究

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Xylanase is a hydrolase enzyme that can hydrolyze hemicellulose into xilo-oligosaccharide and xylose. This research is aimed to investigate the in vitro and in silico xylanase (isolated from the fungus Trichoderma viride ) immobilized on the zeolite matrix activated with HCl 0.4 M solution. The study was performed using in silico docking molecular methods to investigate the interaction between the xylanase primarily to its ligand. Xylanase activity was determined by reducing sugar produced (xylose) by 1 mL of enzyme per minute. The optimum conditions of immobilized xylanase were measured according to the time agitation and concentration of xylanase. The time variation agitation used were 1, 2, 3, 4, and 5 hours, while variations in the concentrations of a xylanase used were 11.500, 15.653, 20.444, 25.875, and 31.944 mg/mL. The optimum conditions of immobilized xylanase was obtained in the shaking time for 3 hours at a concentration of xylanase 15.653 mg/mL and immobilized xylanase activity generated at 46.755 μg.g -1 .min -1 . Immobilized xylanase activity was greater than the purified xylanase (15.976 μg.mL -1 .min -1 ). These results were due to the cofactors Al (AlO 4 ) and Si (SiO 4 ) of zeolite was able to increase the kinetic energy caused the reaction rate between xylanase with the larger substrates. Cofactor also increased the kinetic energy and can enhance the rate of reaction between a xylanase with its substrate, in order to give greater activity. Immobilized xylanase was stable and its reusability as much as 6 times which afforded the activity 21.331 μg.g -1 .min -1 and the efficiency of 56.77%.
机译：木聚糖酶是可以将半纤维素水解为木糖寡糖和木糖的水解酶。这项研究的目的是研究固定在用0.4 M HCl溶液活化的沸石基质上的体外和in silico木聚糖酶（从木霉属木霉菌中分离）。该研究使用计算机对接分子方法进行，以研究木聚糖酶主要与其配体之间的相互作用。通过每分钟将产生的糖（木糖）减少1 mL酶来确定木聚糖酶活性。根据时间搅拌和木聚糖酶的浓度，测定固定化木聚糖酶的最佳条件。所用的时间变化搅动为1、2、3、4和5小时，而所用木聚糖酶浓度的变化为11.500、15.653、20.444、25.875和31.944 mg / mL。在木聚糖酶浓度为15.653 mg / mL的条件下摇动3小时，可获得固定化木聚糖酶的最佳条件，固定化木聚糖酶的活性为46.755μg.g-1 .min -1。固定的木聚糖酶活性大于纯化的木聚糖酶（15.976μg.mL-1 .min -1）。这些结果是由于沸石的辅助因子Al（AlO 4）和Si（SiO 4）能够增加动能而引起木聚糖酶与较大底物之间的反应速率。辅因子还增加了动能，并可以提高木聚糖酶与其底物之间的反应速率，以提供更大的活性。固定化的木聚糖酶是稳定的，其可重复使用性高达6倍，提供的活性为21.331μg.g-1 .min -1，效率为56.77％。

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