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首页> 外文期刊>The Keio Journal of Medicine >REGROWTH ASSAY METHOD FOR QUANTITATIVE EVALUATION OF DRUG-INDUCED CELL DAMAGE IN VITRO: CLARIFICATION OF CELL REDUCTION KINETICS OF ANTICANCER DRUGS AND DIFFERENTIAL DRUG SENSITIVITIES OF GENITOURINARY CANCER CELL LINES
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REGROWTH ASSAY METHOD FOR QUANTITATIVE EVALUATION OF DRUG-INDUCED CELL DAMAGE IN VITRO: CLARIFICATION OF CELL REDUCTION KINETICS OF ANTICANCER DRUGS AND DIFFERENTIAL DRUG SENSITIVITIES OF GENITOURINARY CANCER CELL LINES

机译：体外评估药物诱导的细胞损伤的再生评估方法：澄清抗肿瘤药物的细胞还原动力学和泌尿生殖道肿瘤细胞株的敏感性

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Growth kinetic curves of various human genitourinary cancer cell lines following treatment with an anticancer drug were determined to find a reliable index of cell damage. They showed a triphasic growth pattern consisting of growth inhibition phase, exponential growth phase (regrowth phase) and stationary phase. The percent survival at the turning point from growth inhibition phase to regrowth phase was considered to be a reliable cell damage index indicating to what extent the proliferative capacity of a cell population was impaired by treatment with the drug.Dose-response curves determined by this assay procedure of cell damage (regrowth assay method) clarified the cell reduction kinetics of cis-plati-num (II) diammine dichloride and bleomycin that would provide a rational basis for the mode of their administration in clinical use. They also disclosed the differential drug sensitivities among the cell lines derived from cancers of the different organs represented by HeLa S3, KU 1 and KU 2 and among those from cancers of the same histological type represented by KU 2, NRC 12 and OUR 10 from renal cell carcinoma and KU 1, MGH-U1 and T 24 from transitional cell carcinoma of the bladder. These results suggested that, as the increasing number of human cancer cell lines is established, correlation be-tween drug sensitivity and morphological and functional properties of cancer can be investigated in vitro and the results might provide a rational basis for drug selection.It was concluded that the regrowth assay method to determine drug-induced cell damage in vitro not only has a wide applicability but also is reli-able enough to clarify the cell reduction kinetics of anticancer drugs and the differential drug sensitivities among various genitourinary cancer cell lines.
机译：确定使用抗癌药治疗后各种人类泌尿生殖道癌细胞系的生长动力学曲线，以找到可靠的细胞损伤指数。他们显示出由抑制生长阶段，指数生长阶段（再生阶段）和静止阶段组成的三阶段生长模式。从生长抑制期到再生期的转折点的存活百分率被认为是可靠的细胞损伤指数，表明药物治疗对细胞群的增殖能力有多大程度的损害。细胞损伤的程序（再生试验法）阐明了顺铂（II）二氨二氯化物和博来霉素的细胞减少动力学，这将为它们在临床使用中的给药方式提供合理的依据。他们还揭示了在以HeLa S3，KU 1和KU 2为代表的不同器官的癌症衍生的细胞系以及以KU 2，NRC 12和OUR 10为代表的相同组织学类型的癌症的细胞系中对药物的敏感性差异细胞癌和膀胱移行细胞癌的KU 1，MGH-U1和T 24。这些结果表明，随着建立的人类癌细胞系数量的增加，可以在体外研究药物敏感性与癌症的形态和功能特性之间的相关性，该结果可能为药物选择提供合理的依据。体外测定药物诱导的细胞损伤的再生试验方法不仅具有广泛的适用性，而且其可靠性足以阐明抗癌药物的细胞还原动力学以及各种泌尿生殖道癌细胞系之间的敏感性差异。

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