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首页> 外文期刊>The Journal of the South African Veterinary Association >In vitro investigation of the toxic effects of extracts of Allium sativum bulbs on adults of Hyalomma marginatum rufipes and Rhipicephalus pulchellus
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In vitro investigation of the toxic effects of extracts of Allium sativum bulbs on adults of Hyalomma marginatum rufipes and Rhipicephalus pulchellus

机译：大蒜鳞茎提取物对边缘透明质红球菌和深头hip的成虫的体外毒性研究

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摘要

The toxic effects of the extracts of Allium sativum (Garlic) were evaluated against adults of Hyalomma marginatum rufipes and Rhipicephalus pulchellus using three types (Types A, B and C) of contact toxicity bioassays. A. sativum bulbs were extracted with acetone, ethanol and dichloromethane (DCM) solvents. Among these three solvents, it is the DCM extract of A. sativum that appears to have anti-tick activity. In the Type A contact toxicity bioassay, DCM extracts of A. sativum demonstrated a high acaricidal bioactivity against H. m. rufipes with 100 % of ticks killed in less than an hour, and toxicity persisted to the second day. A weak acaricidal activity of aqueous extracts of A. sativum was observed in the Type B contact toxicity bioassay. In the Type C contact toxicity bioassay, a concentration of 24 % w/v of DCM extracts of garlic in sunflower oil (Helianthus annuus) had killed 100% of H. m. rufipes (LC50= 5.9 % w/v) and R. pulchellus (LC50 = 10.3 % w/v) by 24 hours post-treatment of ticks. The results obtained from this study suggest that DCM extract of A. sativum is a potential source of novel acaricidal agents.
机译：使用三种类型（A，B和C型）接触毒性生物测定法评估了大蒜（大蒜）提取物对边缘透明质红球菌和深头鼻吸虫的成虫的毒性作用。用丙酮，乙醇和二氯甲烷（DCM）溶剂萃取番茄球茎。在这三种溶剂中，似乎是具有抗t虫活性的是农杆菌的DCM提取物。在A型接触毒性生物测定中，农杆菌的DCM提取物对H. m具有很高的杀螨生物活性。在不到一个小时的时间内杀死了100％s虫的红斑痣，毒性一直持续到第二天。在B型接触毒性生物测定法中，观察到了A. sativum的水提物的弱杀螨活性。在C型接触毒性生物测定中，葵花籽油（Helianthus annuus）中大蒜DCM提取物浓度为24％w / v已杀死100％的H. m。 s虫治疗后24小时，rufipes（LC50 = 5.9％w / v）和R. pulchellus（LC50 = 10.3％w / v）。从这项研究中获得的结果表明，农杆菌的DCM提取物是新型杀螨剂的潜在来源。

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