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首页> 外文期刊>The Open Anesthesia Journal >Isoflurane but not Fentanyl Causes Apoptosis in Immature Primary Neuronal Cells
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Isoflurane but not Fentanyl Causes Apoptosis in Immature Primary Neuronal Cells

机译：异氟烷而非芬太尼引起未成熟原代神经元细胞凋亡

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Background:Anaesthetics are widely used in new-borns and preterm infants, although it is known that they may adversely affect the developing brain.Objective:We assessed the impact of the volatile anaesthetic, isoflurane, and the intravenous analgesic, fentanyl, on immature and mature embryonic neuronal cells.Methods:Primary neuronal cultures from embryonic rats (E18) cultured for 5 (immature) or 15 days (mature) in vitro (DIV), respectively, were exposed to isoflurane (1.5 Vol.%) or fentanyl (0.8 - 200 ng/ml) for 24 hours. Experiments were repeated in the presence of the γ-amino butyric acid-A (GABA_(A)) receptor antagonists, bicuculline or picrotoxin (0.1 mmol/l), or the pancaspase inhibitor zVAD-fmk (20 nmol/l). Cell viability was assessed by methyltetrazolium (MTT) metabolism or lactate dehydrogenase (LDH) release.Results:Isoflurane reduced cell viability significantly in primary neuronal cells cultured for 5 DIV (Δ MTT -28 ±13%, Δ LDH +143 ±15%). Incubation with bicuculline, picrotoxin or zVAD-fmk protected the cells mostly from isoflurane toxicity. After 15 DIV, cell viability was not reduced by isoflurane. Viability of primary neurons cultured for 5 DIV did not change with fentanyl over the wide range of concentrations tested.Conclusion:Immature primary neurons may undergo apoptosis following exposure to isoflurane but are unaffected by fentanyl. Mature primary neurons were not affected by isoflurane exposure.
机译：背景：麻醉药已广泛用于新生儿和早产儿，尽管已知它们可能会对发育中的大脑造成不利影响。方法：将分别在体外（DIV）培养5天（未成熟）或15天（成熟）的胚胎大鼠（E18）的原始神经元培养物暴露于异氟烷（1.5 Vol。％）或芬太尼（0.8 -200 ng / ml），持续24小时。在γ-氨基丁酸-A（GABA_（A））受体拮抗剂，双小分子或微毒素（0.1 mmol / l）或泛半胱氨酸蛋白酶抑制剂zVAD-fmk（20 nmol / l）的存在下重复实验。通过甲基四氮唑（MTT）代谢或乳酸脱氢酶（LDH）释放评估细胞活力。结果：异氟烷显着降低了培养5 ​​DIV的原代神经元细胞的细胞活力（ΔMTT -28±13％，ΔLDH +143±15％） 。与小分子，苦瓜毒素或zVAD-fmk一起温育可保护细胞免受异氟烷的毒性。 15 DIV后，异氟烷不会降低细胞活力。在不同浓度的芬太尼中，培养5 DIV的原代神经元的活力并未改变。结论：未成熟的原代神经元在暴露于异氟烷后可能会发生凋亡，但不受芬太尼影响。成熟的原代神经元不受异氟烷暴露的影响。

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