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首页> 外文期刊>Hepatic Medicine: Evidence and Research >Relationship between serum HBsAg level, HBV DNA level, and peripheral immune cells in patients with chronic hepatitis B virus infection
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Relationship between serum HBsAg level, HBV DNA level, and peripheral immune cells in patients with chronic hepatitis B virus infection

机译：慢性乙型肝炎病毒感染患者血清HBsAg，HBV DNA水平与外周免疫细胞的关系

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Background: The chronicity of hepatitis B virus (HBV) infection is attributed to inappropriate functioning of cell-mediated immunity. Besides the importance of measuring serum HBV DNA and HBV surface antigen (HBsAg) as markers of viral replication and exposure, respectively, studies regarding their influence on immune cell status in chronic HBV infection are still scarce. Because such studies of chronic HBV patients have not been reported for India, we attempted to evaluate the relationship between serum concentrations of HBsAg, HBV DNA, and percentage of immune cells in peripheral blood of Indian subjects with chronic HBV infection.Methods: Thirty-one HbsAg-positive subjects were evaluated for serum HBe antigen (HBeAg), anti-Hbe, and alanine transferase status by standard enzyme-linked immunosorbent assay (ELISA) and biochemical procedures. Serum HBV DNA level was determined by real-time TaqMan? polymerase chain reaction assay. Serum HBsAg level was measured by a third-generation sandwich ELISA kit. Peripheral immune cell profiling was done by multifluorometric flow cytometry analysis, for which 21 healthy subjects were included as controls.Results: The majority (93.5%) of the study subjects were HBeAg-negative and anti-HBeAg-positive. Mean viral load, HBsAg, and alanine transferase levels were 4.20 ± 1.96 log copies/mL, 5.98 ± 4.62 log IU/mL, and 74.5 ± 110 IU/mL, respectively. In comparison with controls, total T cell and cytotoxic T cell populations were significantly (P < 0.05) reduced in HBV-infected subjects, while the status of B cells, natural killer cells, T helper cells, and ratio of T helper to cytotoxic cells remained unaltered.Conclusion: Suppression of the peripheral cytotoxic T cell population in chronic HBeAg-negative chronic HBV infection is influenced by increased viral load. Serum HBsAg concentration appeared independent of serum HBV DNA level and immune cell status. Nonelevation of natural killer cell and T helper cell numbers in subjects harboring lower to moderate HBV loads is further indicative of noninduction of innate as well as a coordinated adaptive immune response favoring chronicity of the disease.
机译：背景：乙型肝炎病毒（HBV）感染的长期性归因于细胞介导的免疫功能异常。除了分别测量血清HBV DNA和HBV表面抗原（HBsAg）作为病毒复制和暴露的标志物的重要性外，关于它们对慢性HBV感染中免疫细胞状态影响的研究仍然很少。由于印度尚无关于慢性HBV患者的此类研究的报道，我们试图评估慢性HBV感染印度受试者血清HBsAg，HBV DNA浓度与外周血免疫细胞百分比之间的关系。方法：31通过标准酶联免疫吸附测定（ELISA）和生化程序评估HbsAg阳性受试者的血清HBe抗原（HBeAg），抗Hbe和丙氨酸转移酶状态。血清HBV DNA水平是通过实时TaqMan？聚合酶链反应测定。血清HBsAg水平通过第三代三明治ELISA试剂盒测量。通过多荧光流式细胞仪分析进行外周免疫细胞谱分析，其中包括21名健康受试者作为结果。结果：研究受试者中的大多数（93.5％）为HBeAg阴性和抗HBeAg阳性。平均病毒载量，HBsAg和丙氨酸转移酶水平分别为4.20±1.96 log IU / mL，5.98±4.62 log IU / mL和74.5±110 IU / mL。与对照组相比，HBV感染者的总T细胞和细胞毒性T细胞群体显着减少（P <0.05），而B细胞，自然杀伤细胞，T辅助细胞的状态以及T辅助细胞与细胞毒性细胞的比率结论：慢性HBeAg阴性慢性HBV感染中外周细胞毒性T细胞的抑制受病毒载量增加的影响。血清HBsAg浓度与血清HBV DNA水平和免疫细胞状态无关。携带较低至中度HBV负荷的受试者中自然杀伤细胞和T辅助细胞数量的未升高进一步指示了先天性的不诱导以及协调的适应性免疫应答，有利于疾病的慢性发展。

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