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Adaptation ofEscherichia coliATCC 8739 to 11% NaCl

机译:大肠杆菌ATCC 8739对11%NaCl的适应性

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Escherichia coli(E. coli) is a nonhalophilic microbe and used to indicate faecal contamination. Salt (sodium chloride, NaCl) is a common food additive and is used in preservatives to encounter microbial growth. The effect of howE. coliinteracts with the salt present in the human diet is unclear. Thus, it is important to investigate this relationship. In order to adapt and survive the changes in the environment,E. colimay undergo halophilization. In this study, we observed the genetic changes and growth kinetics ofE. coliATCC 8739 under 3%–11% NaCl over 80 passages. Our results suggest thatE. coliadapted to 1% increase in NaCl every month with a successful adaptation to 11% NaCl. Gram staining and PCR/RFLP showed that the cultures are Gram negative and the DNA profiles of all 4 replicates to be similar, suggesting that the cultures had not been contaminated.
机译:大肠杆菌(E. coli)是非嗜盐微生物,用于指示粪便污染。盐(氯化钠,NaCl)是常见的食品添加剂,用于防腐​​剂时会遇到微生物的生长。 howE的效果。大肠杆菌与人类饮食中存在的盐是否相互作用尚不清楚。因此,研究这种关系很重要。为了适应和生存环境的变化,E.大肠菌可能会进行冻干。在这项研究中,我们观察到了E的遗传变化和生长动力学。在80遍传代过程中,在3%–11%NaCl中使用coliATCC 8739。我们的结果表明。大肠杆菌成功适应了11%的NaCl,每月使NaCl的含量增加了1%。革兰氏染色和PCR / RFLP显示培养物为革兰氏阴性,并且所有4个重复的DNA图谱均相似,表明培养物未受到污染。

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