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首页> 外文期刊>Drug Design, Development and Therapy >Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in? microaerophilic Giardia lamblia
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Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in? microaerophilic Giardia lamblia

机译：氧化应激诱导的细胞周期阻滞和不依赖蛋白酶的程序性细胞死亡？微需氧贾第鞭毛虫

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Abstract: Giardia lamblia is a microaerophilic human gastrointestinal parasite and considered as an early-diverged eukaryote. In vitro oxidative stress generation plays a significant role in cell cycle progression and cell death of this parasite. In the present study hydrogen peroxide, metronidazole, and a modified growth medium without cysteine and ascorbic acid have been chosen as oxidative stress-inducing agents. Cell cycle progression has been found to be regulated by different types of oxidative stresses. Apoptosis is not an established pathway in Giardia, which is devoid of ideal mitochondria, but in the present investigation, apoptosis-like programmed cell death has been found by the experiments like AnnexinV-FITC assay, DNA fragmentation pattern, etc. On the contrary, Caspase-9 assay, which confirms the caspase-mediated apoptotic pathway, has been found to be negative in all the stress conditions. Protease inhibitor assay confirmed that, even in absence of any proteases, programmed cell death does occur in this primitive eukaryote. All these results signify a novel pathway of programmed suicidal death in Giardia lamblia under oxidative stress. This is the first demonstration of protease-independent programmed cell death regulation in Giardia exclusive for its own specialties.
机译：摘要：贾第鞭毛虫是一种微需氧的人胃肠道寄生虫，被认为是早发的真核生物。体外氧化应激的产生在该寄生虫的细胞周期进程和细胞死亡中起重要作用。在本研究中，过氧化氢，甲硝唑和不含半胱氨酸和抗坏血酸的改良生长培养基已被选作氧化应激诱导剂。已经发现细胞周期进程受不同类型的氧化应激调节。凋亡不是贾第鞭毛虫的既定途径，缺乏理想的线粒体，但是在本研究中，通过膜联蛋白V-FITC测定，DNA片段化模式等实验发现了凋亡样程序性细胞死亡。 Caspase-9分析法证实了caspase介导的凋亡途径，在所有应激条件下均呈阴性。蛋白酶抑制剂测定证实，即使不存在任何蛋白酶，在该原始真核生物中也确实发生了程序性细胞死亡。所有这些结果表明在氧化应激下贾第鞭毛虫的程序性自杀死亡的新途径。这是贾第鞭毛虫中不依赖于蛋白酶的程序性细胞死亡调控的第一个证明，它专有于自身。

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