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首页> 外文期刊>Journal of Applied Life Sciences International >Larvicidal Activities of N-Hexane Fraction of Ocimum gratissimum Leaf against Mosquito Larvae and Its GC-Ms Analysis of Phytoconstituents
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Larvicidal Activities of N-Hexane Fraction of Ocimum gratissimum Leaf against Mosquito Larvae and Its GC-Ms Analysis of Phytoconstituents

机译：香茅叶正己烷级分对蚊虫幼虫的杀幼虫活性及其植物成分的GC-MS分析

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Aim: To evaluate the larvicidal efficacy and identify the active phytochemical of Ocimum gratissimum n-hexane leaf extract on mosquito larvae. Study Design: Reflux extraction, standard qualitative phytochemical tests, Column and Thin layer chromatographic methods and GC- MS analysis. Place and Duration of Study: Department of Biochemistry, STEP-B (Genetic Engineering) Laboratory, Federal University of Technology, Minna between August, 2013 and July 2014. Methodology: The plant leaves was extracted with 70% methanol, crude extract was partitioned into fractions and healthy mosquito larvae subjected to Standard WHO insecticide susceptibility protocol for the extract efficacy. Column and Thin layer Chromatographic methods were used for phyto-constituent separation of plant extract. The most effective larvicidal column chromatography fraction was subjected to GC-MS analysis for further quantitative and identification of the most effective phytochemical isolates. The phytochemical constituents were determined qualitatively. Results: Maximum larvae mortality (100%) was observed in 2, 4 and 8% w/v concentrations of leaf extract of O. gratissimum 24 h post exposure. Column chromatographic fraction F42 gave 100% larvicidal activity at 0.5% concentration. The predominant phytochemical constituents detected in the leaf extract of this plant were terpenes, saponins and tannins. Steroids and phenols were detected in all extracts but absent in the ethanolic leaf extracts. Flavonoids were not detected in any extracts of the plant. The GC-MS analysis of fraction F42 from O. gratissimum reflected thymol, caryophyllene and terpenoids were present. Conclusion: The study clearly indicates that O. gratissimum possesses chemical compounds that are active against mosquito larvae. Therefore these chemicals can be formulated into mosquito larvicides to prevent mosquito borne diseases.
机译：目的：为了评估杀幼虫剂的功效，并鉴定出Ocimum gratissimum n-己烷叶提取物对蚊虫幼虫的活性植物化学成分。研究设计：回流萃取，标准定性植物化学测试，柱和薄层色谱方法以及GC-MS分析。研究的地点和时间：2013年8月至2014年7月，在明尼苏达州联邦技术大学STEP-B（基因工程）实验室生物化学系。方法：用70％甲醇提取植物叶片，将粗提物分配到馏分和健康的蚊虫幼虫要接受标准的WHO杀虫剂敏感性试验，以确保提取物的有效性。柱色谱法和薄层色谱法用于植物提取物的植物成分分离。对最有效的幼虫柱色谱部分进行GC-MS分析，以进一步定量和鉴定最有效的植物化学分离物。定性确定了植物化学成分。结果：暴露后24小时，O。gratissimum的叶提取物的2、4和8％w / v浓度观察到最大幼虫死亡率（100％）。柱色谱馏分F 42 在0.5％的浓度下具有100％的杀幼虫活性。在该植物的叶提取物中检测到的主要植物化学成分是萜烯，皂苷和单宁酸。在所有提取物中均检测到类固醇和苯酚，但在乙醇性叶提取物中未检测到。在植物的任何提取物中均未检出类黄酮。用气相色谱-质谱法分析了来自O. gratissimum的馏分F 42 的含量，结果表明存在百里香酚，石竹烯和萜类化合物。结论：该研究清楚地表明，O。gratissimum具有对蚊子幼虫具有活性的化学化合物。因此，可以将这些化学物质配制成蚊虫幼虫，以预防蚊媒传播的疾病。

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