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首页> 外文期刊>Journal of genetics >Complete sequence of the mitochondrial genome of Odontamblyopus rubicundus (Perciformes: Gobiidae): genome characterization and phylogenetic analysis
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Complete sequence of the mitochondrial genome of Odontamblyopus rubicundus (Perciformes: Gobiidae): genome characterization and phylogenetic analysis

机译：Odontamblyopus rubicundus（Perciformes：Gobiidae）的线粒体基因组的完整序列：基因组表征和系统发育分析

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摘要

Odontamblyopus rubicundus is a species of gobiid fishes, inhabits muddy-bottomed coastal waters. In this paper, the first complete mitochondrial genome sequence of O. rubicundus is reported. The complete mitochondrial genome sequence is 17119 bp in length and contains 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a control region and an L-strand origin as in other teleosts. Most mitochondrial genes are encoded on H-strand except for ND6 and seven tRNA genes. Some overlaps occur in protein-coding genes and tRNAs ranging from 1 to 7 bp. The possibly nonfunctional L-strand origin folded into a typical stem-loop secondary structure and a conserved motif (5a€2-GCCGG-3a€2) was found at the base of the stem within the $tRNA^{Cys}$ gene. The TAS, CSB-2 and CSB-3 could be detected in the control region. However, in contrast to most of other fishes, the central conserved sequence block domain and the CSB-1 could not be recognized in O. rubicundus, which is consistent with Acanthogobius hasta (Gobiidae). In addition, phylogenetic analyses based on different sequences of species of Gobiidae and different methods showed that the classification of O. rubicundus into Odontamblyopus due to morphology is debatable.
机译：Odontamblyopus rubicundus是一种戈壁鱼，栖息在浑浊的沿海水域。本文报道了O. rubicundus的第一个完整的线粒体基因组序列。完整的线粒体基因组序列长度为17119 bp，与其他硬骨鱼一样，包含13个蛋白质编码基因，两个rRNA基因，22个tRNA基因，一个控制区和一个L链起源。除ND6和7个tRNA基因外，大多数线粒体基因均在H链上编码。在1至7 bp的蛋白质编码基因和tRNA中出现了一些重叠。可能无功能的L链起源折叠成典型的茎环二级结构，并且在$ tRNA ^ {Cys} $基因内茎的基部发现了保守的基序（5a€2-GCCGG-3a€2）。可以在控制区域中检测到TAS，CSB-2和CSB-3。但是，与大多数其他鱼类相反，在O. rubicundus中无法识别出中央保守序列区块结构域和CSB-1，这与Acanthogobius hasta（Gobiidae）一致。此外，根据不同的戈壁科物种序列和不同的方法进行的系统发育分析表明，由于形态学的原因，将O. rubicundus分类为Odontamblyopus是值得商bat的。

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