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首页> 外文期刊>Journal of Hainan Medical University >Value of HMGA2, Tiam1 and Notch1 expression for evaluating the malignant degree of non-small cell lung cancer
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Value of HMGA2, Tiam1 and Notch1 expression for evaluating the malignant degree of non-small cell lung cancer

机译：HMGA2，Tiam1和Notch1表达在评估非小细胞肺癌恶性程度中的价值

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Objective: To study the evaluation value of HMGA2, Tiam1 and Notch1 expression forcancer cell proliferation, invasion and epithelial-mesenchymal transition in non-small celllung cancer tissue. Methods: Non-small cell lung cancer tissues and para-carcinoma tissuessurgically removed in our hospital between May 2013 and December 2015 were collected,immunohistochemical staining method was used to determine HMGA2, Tiam1 and Notch1protein expression, and fluorescence quantitative PCR was used to determine the mRNAexpression of HMGA2, Tiam1, Notch1 as well as proliferation, invasion and epithelialmesenchymaltransition molecules. Results: HMGA2, Tiam1 and Notch1 mRNA expressionand positive protein expression rate in lung cancer tissues were significantly higher than thosein para-carcinoma tissues (P0.05); E-cadherin mRNA expression in lung cancer tissues withpositive HMGA2 expression was significantly lower than that in lung cancer tissues withnegative HMGA2 expression (P0.05) while N-cadherin and Vimentin mRNA expressionwere significantly higher than those in lung cancer tissues with negative HMGA2 expression(P0.05); MMP2, MMP3, MMP8 and MMP9 mRNA expression in lung cancer tissues withpositive Tiam1 expression were significantly higher than those in lung cancer tissues withnegative Tiam1 expression (P0.05); CyclinD1, Bcl-2 and Survivin mRNA expression in lungcancer tissues with positive Notch1 expression were significantly higher those in lung cancertissues with negative Notch1 expression (P0.05). Conclusions: Highly expressed HMGA2,Tiam1 and Notch1 in lung cancer tissues can regulate the epithelial-mesenchymal transition,invasion and proliferation of cancer cells respectively.
机译：目的：研究HMGA2，Tiam1和Notch1表达对非小细胞肺癌组织癌细胞增殖，侵袭和上皮间质转化的评价价值。方法：收集2013年5月至2015年12月在我院手术切除的非小细胞肺癌组织和癌旁组织，采用免疫组织化学染色法检测HMGA2，Tiam1和Notch1蛋白的表达，并采用荧光定量PCR检测HMGA2，Tiam1，Notch1的mRNA表达以及增殖，侵袭和上皮间质转化分子。结果：肺癌组织中HMGA2，Tiam1和Notch1的mRNA表达和阳性蛋白表达率显着高于癌旁组织（P <0.05）。 HMGA2表达阳性的肺癌组织中E-cadherin mRNA的表达明显低于HMGA2表达阴性的肺癌组织中E-cadherin mRNA的表达（P <0.05），而HMGA2表达阴性的肺癌组织中E-cadherin和Vimentin mRNA的表达显着更高（ P <0.05）; Tiam1表达阳性的肺癌组织中MMP2，MMP3，MMP8和MMP9 mRNA的表达显着高于Tiam1表达阴性的肺癌组织中（P <0.05）; Notch1表达阳性的肺癌组织中CyclinD1，Bcl-2和Survivin mRNA的表达明显高于Notch1表达阴性的肺癌组织中的CyclinD1，Bcl-2和Survivin mRNA表达（P <0.05）。结论：在肺癌组织中高表达的HMGA2，Tiam1和Notch1分别调节癌细胞的上皮-间质转化，侵袭和增殖。

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