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首页> 外文期刊>Journal of Molecular Biochemistry >Cloning and functional characterization of a vertebrate low-density lipoprotein receptor homolog from eri silkmoth, Samia ricini
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Cloning and functional characterization of a vertebrate low-density lipoprotein receptor homolog from eri silkmoth, Samia ricini

机译：蚕（Samia ricini）的脊椎动物低密度脂蛋白受体同源物的克隆与功能鉴定

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摘要

The lipophorin receptor (LpR) is the insect lipoprotein receptor and belongs to the low-density lipoprotein receptor (LDLR) superfamily. It has a vital role in the uptake of lipophorin (Lp) into various tissues. Here we report the full length cloning and functional characterization of an LpR from eri silkmoth, Samia ricini . The full length cDNA of SrLpR7-1 is 4132 bp including an open reading frame (ORF) of 2595 bp. The deduced amino acid sequence revealed well structured ligand binding, epidermal growth factor, glycosylation, transmembrane and cytoplasmic domains. The ligand binding domain consisted of seven cysteine repeats instead of the common eight cysteine repeats indicating it as a homolog of human LDLR. We identified another splice variant, Sr LpR7-2 with a deletion of 27 amino acids in the O-glycosylation domain. Apart from the fat body, both isoforms are expressed in ovary, brain and other tissues at different developmental stages of the silkworm. RNAi experiments did not show any marked effects except that the adult emergence was delayed compared to controls. In addition, the SrLpR7 cDNA was recombinantly expressed and ligand binding experiments confirmed that the receptor protein binds not only to Sr Lp but also to Bombyx mori Lp.
机译：脂蛋白受体（LpR）是昆虫脂蛋白受体，属于低密度脂蛋白受体（LDLR）超家族。它在各种组织摄取脂蛋白（Lp）中起着至关重要的作用。在这里，我们报道了来自蚕蛾（Samia ricini）的LpR的全长克隆和功能表征。 SrLpR7-1的全长cDNA为4132 bp，包括2595 bp的开放阅读框（ORF）。推导的氨基酸序列揭示了结构良好的配体结合，表皮生长因子，糖基化，跨膜和细胞质结构域。配体结合结构域由七个半胱氨酸重复组成，而不是由常见的八个半胱氨酸重复组成，表明它是人LDLR的同源物。我们确定了另一个剪接变体，Sr LpR7-2与O-糖基化域中的27个氨基酸的缺失。除脂肪体外，两种同工型均在家蚕不同发育阶段的卵巢，大脑和其他组织中表达。 RNAi实验没有显示任何明显的作用，只是与对照相比，成年人的出现有所延迟。此外，SrLpR7 cDNA被重组表达，配体结合实验证实该受体蛋白不仅与Sr Lp结合，而且与Bombyx mori Lp结合。

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