首页> 外文期刊>Journal of Molecular Biology Research >Assessing Polymorphism Information Content (PIC) Using SSR Molecular Markers on Local Species of Citrullus Colocynthis. Case Study: Iran, Sistan-Balouchestan Province
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Assessing Polymorphism Information Content (PIC) Using SSR Molecular Markers on Local Species of Citrullus Colocynthis. Case Study: Iran, Sistan-Balouchestan Province

机译:使用SSR分子标记对柑桔本地物种的多态性信息含量(PIC)进行评估。案例研究:伊朗锡斯坦-Balouchestan省

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Studying polymorphism information content in plants, particularly local plants which are rich in genetic variety, can play an efficient role in building genetic bank and species’ breeding. Iranian colocynth is highly important in terms of medicinal and therapeutic traits and hence, it needs assessing the polymorphism information content. In present research, simple sequence repeat (SSR) markers are used. 32 samples are randomly collected from local accumulations in 8 regions of Sistan-Balouchestan province. DNA extraction from each sample was done using Cetyltrimethylammonium Bromide. 10 primers were designed and used in this study. Polymerase chain reaction was done using extracted DNA and ten primers. To analyze genetic data, NTSYS pc ver.2/2, Genalex 6.5 and XLSATAT software were used. Regarding data analysis of respective values and taking this fact into account that7 out of 10 used primers showed polymorphism information content in respective samples, their classification was not possible and hence, categorization of similar shapes was not performed due to highly genetic diversity of this species and, also, each value had a different shape.
机译:研究植物,特别是具有丰富遗传多样性的本地植物中的多态性信息含量,可以在建立遗传库和物种育种方面发挥有效作用。伊朗co鱼在药物和治疗特性方面非常重要,因此需要评估其多态性信息含量。在本研究中,使用了简单的序列重复(SSR)标记。从Sistan-Balouchestan省的8个地区的当地堆积物中随机采集了32个样本。使用十六烷基三甲基溴化铵从每个样品中提取DNA。设计了10个引物并用于本研究。使用提取的DNA和十个引物进行聚合酶链反应。为了分析遗传数据,使用了NTSYS pc ver.2 / 2,Genalex 6.5和XLSATAT软件。关于各个值的数据分析,并考虑到以下事实:10个引物中有7个在各个样本中显示多态性信息含量,无法对其进行分类,因此,由于该物种的高度遗传多样性,因此未进行相似形状的分类。 ,而且,每个值都有不同的形状。

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