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首页> 外文期刊>Journal of Oral Microbiology >Microcosm biofilms cultured from different oral niches in periodontitis patients
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Microcosm biofilms cultured from different oral niches in periodontitis patients

机译：牙周炎患者不同口腔发育的缩微生物膜

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ABSTRACT Objective : Periodontal diseases are triggered by dysbiotic microbial biofilms. Therefore, it is essential to develop appropriate biofilm models. Aim of the present study was to culture microcosm biofilms inoculated from different niches in periodontitis patients and compare their microbial composition to those inoculated from subgingival plaque. Methods : Saliva, subgingival plaque, tongue and tonsils were sampled in five periodontitis patients to serve as inocula for culturing biofilms in vitro in an active attachment model. Biofilms were grown for 14 or 28 d and analyzed for their microbial composition by 16S rDNA sequencing. Results : As classified by HOMD, all biofilms were dominated by periodontitis-associated taxa, irrespective which niche had been used for inoculation. There was a low similarity between 14 d biofilms and their respective inocula (Bray-Curtis similarity 0.26), while biofilms cultured for 14 and 28 d shared high similarity (0.69). Principal components analysis showed much stronger clustering per patient than per niche indicating that the choice of patients may be more crucial than choice of the respective niches in these patients. Conclusion : Saliva, tongue scrapings or tonsil swabs may represent sufficient alternative inocula for growing microcosm biofilms resembling periodontitis-associated microbial communities in cases when sampling subgingival plaque is not possible.
机译：摘要目的：牙周疾病是由生物不良的微生物生物膜引发的。因此，开发合适的生物膜模型至关重要。本研究的目的是在牙周炎患者中培养接种自不同部位的缩微生物膜，并将其微生物成分与从牙龈下斑块接种的微生物膜进行比较。方法：对5例牙周炎患者的唾液，龈下菌斑，舌头和扁桃体进行取样，作为接种物，以主动贴壁模型体外培养生物膜。生物膜生长14或28天，并通过16S rDNA测序分析其微生物组成。结果：按照HOMD分类，所有生物膜均以牙周炎相关的分类单元为主导，无论使用哪种利基进行接种。 14 d生物膜和它们各自的接种物之间的相似性很低（Bray-Curtis相似性0.26），而培养14和28 d的生物膜具有很高的相似性（0.69）。主成分分析显示，每个患者的聚类要比每个利基要强得多，这表明在这些患者中，患者的选择可能比选择相应壁ni更为关键。结论：在无法取样龈下菌斑的情况下，唾液，刮擦舌头或扁桃体拭子可能是生长类似于微生物的牙周炎相关微生物群落的足够的替代性接种物。

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