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首页> 外文期刊>Journal of Pharmaceutical Analysis >Synthesis of curcuminoid-imprinted polymers applied to the solid-phase extraction of curcuminoids from turmeric samples
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Synthesis of curcuminoid-imprinted polymers applied to the solid-phase extraction of curcuminoids from turmeric samples

机译：姜黄素印迹聚合物的合成，用于从姜黄样品中固相萃取姜黄素

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A molecular imprinting polymer technique was successfully applied to precipitation polymerization by using styrene as a functional monomer, curcuminoids as templates, acetonitrile as a porogenic solvent, benzoyl peroxide as the initiator, and ethylene glycol dimethacrylate as the crosslinker. The effects of interaction on the adsorption capacity of the molecularly imprinted polymer (MIP) and non-imprinted polymer (NIP) were investigated. A comparison of the adsorption capacity for MIP and NIP indicated that the NIP had the lowest adsorption capacity. The curcuminoid-imprinted polymer (Cur-MIP) was synthesized from 0.0237mmol of styrene, 47.0g of acetonitrile, 1.0238mmol of ethylene glycol dimethacrylate, 0.0325mmol of curcuminoids, and 0.2480mmol of benzoyl peroxide. A high-performance liquid chromatography method with fluorescence detection was developed and validated for various chromatographic conditions for the determination of the curcuminoids in turmeric samples. The sample solution was separated using the Cur-MIP via solid-phase extraction and analyzed on a Brownlee analytical C 18 column (150mm × 6mm, 5μm) using an isocratic elution consisting of acetonitrile and 0.1% trichloroacetic acid (40:60, v/v). The flow rate was maintained at 1.5mL/min. The fluorescence detector was set to monitor at λ ex = 426nm and λ em = 539nm. The quantification limit values were found to be 16.66, 66.66, and 33.33μg/L for curcumin, demethoxycurcumin, and bisdemethoxycurcumin, respectively. Thus, we concluded that the Cur-MIP and high-performance liquid chromatographic-fluorescence method could be applied to selective extraction and could be used as a rapid tool for the determination of curcuminoids in medicinal herbal extracts.
机译：通过使用苯乙烯作为功能单体，使用姜黄素类作为模板，使用乙腈作为致孔剂，使用过氧化苯甲酰作为引发剂以及使用乙二醇二甲基丙烯酸酯作为交联剂，将分子印迹聚合物技术成功应用于沉淀聚合。研究了相互作用对分子印迹聚合物（MIP）和非印迹聚合物（NIP）吸附容量的影响。对MIP和NIP的吸附容量的比较表明，NIP具有最低的吸附容量。由0.0237mmol的苯乙烯，47.0g的乙腈，1.0238mmol的乙二醇二甲基丙烯酸酯，0.0325mmol的姜黄素和0.2480mmol的过氧化苯甲酰合成了姜黄素印迹的聚合物（Cur-MIP）。开发了一种具有荧光检测功能的高效液相色谱方法，并已针对各种色谱条件进行了验证，以确定姜黄样品中的姜黄素含量。使用Cur-MIP通过固相萃取分离样品溶液，并在Brownlee分析C 18色谱柱（150mm×6mm，5μm）上进行分析，使用由乙腈和0.1％三氯乙酸组成的等度洗脱液（40：60，v / v）。流速保持在1.5mL / min。荧光检测器设置为在λex = 426nm和λem = 539nm处监测。姜黄素，去甲氧基姜黄素和双去甲氧基姜黄素的定量极限分别为16.66、66.66和33.33μg/ L。因此，我们得出的结论是，Cur-MIP和高效液相色谱-荧光方法可用于选择性提取，并可作为测定草药提取物中姜黄素的快速工具。

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