• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Young Pharmacists >Arginase Inhibition Activity of Stem Bark Extract of Caesalpinia pulcherrima
【24h】

Arginase Inhibition Activity of Stem Bark Extract of Caesalpinia pulcherrima

机译：es草茎皮提取物的精氨酸酶抑制活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Inhibition of arginase activity can ameliorate endothelial dysfunction in vascular system which can cause some cardiovascular diseases. This study was aimed to test the arginase activity inhibition of n-hexane, ethyl acetate, and methanol extract of C. pulcherrima (L.) Sw. stem bark and determine total flavonoid content of the most active extract. Methods: C. pulcherrima (L.) Sw. stem bark was extracted using multistage reflux. In vitro arginase activity inhibition was done using 1 U/mL enzyme concentration and 570 mM substrate concentration. The product was measured by microplate reader of wavelength 430 nm. Results: Methanolic extract showed to be the most active extract in the test. IC50 value of methanolic extract was 21.969 μg/mL which was higher than standard N(omega)-hydroxy-nor-L-arginine acetate (nor-NOHA acetate) that had IC50 value of 3.994 μg/mL. The result of total flavonoid content determination of methanol extract by colorimetric AlCl3 was 3.943 mgQE/gram extract. Phytochemical screening shows that methanol extract of C. pulcherrima (L.) Sw. stem bark contains flavonoids, tannins, saponins, and triterpenoids. Conclusion: Methanol extract of C. pulcherrima (L.) Sw. had significant inhibitory activity of arginase and can be used as a potential source for the development of arginase inhibitor.
机译：背景：精氨酸酶活性的抑制可以改善血管系统的内皮功能障碍，从而导致某些心血管疾病。这项研究的目的是测试对C. pulcherrima（L.）Sw。的正己烷，乙酸乙酯和甲醇提取物精氨酸酶活性的抑制作用。茎皮，并确定活性最高的提取物中总黄酮含量。方法：C。pulcherrima（L.）Sw。使用多级回流提取茎皮。使用1 U / mL酶浓度和570 mM底物浓度进行体外精氨酸酶活性抑制。用酶标仪检测波长为430 nm的产物。结果：甲醇提取物显示出是测试中活性最高的提取物。甲醇提取物的IC50值为21.969μg/ mL，高于标准的N（ω）-羟基-nor-L-精氨酸乙酸酯（nor-NOHA乙酸盐）的IC50值为3.994μg/ mL。比色AlCl3测定甲醇提取物的总黄酮含量为3.943 mgQE /克提取物。植物化学筛选显示，C。pulcherrima（L.）Sw。的甲醇提取物。茎皮中含有类黄酮，单宁，皂苷和三萜。结论：C. pulcherrima（L.）Sw。的甲醇提取物。具有精氨酸酶的显着抑制活性，可作为开发精氨酸酶抑制剂的潜在来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号