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首页> 外文期刊>BMC Genomics >Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence
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Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence

机译：食蟹猴睾丸cDNA用于人类基因组序列中新人类基因的发现

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Background In order to contribute to the establishment of a complete map of transcribed regions of the human genome, we constructed a testicular cDNA library for the cynomolgus monkey, and attempted to find novel transcripts for identification of their human homologues. Result The full-insert sequences of 512 cDNA clones were determined. Ultimately we found 302 non-redundant cDNAs carrying open reading frames of 300 bp-length or longer. Among them, 89 cDNAs were found not to be annotated previously in the Ensembl human database. After searching against the Ensembl mouse database, we also found 69 putative coding sequences have no homologous cDNAs in the annotated human and mouse genome sequences in Ensembl. We subsequently designed a DNA microarray including 396 non-redundant cDNAs (with and without open reading frames) to examine the expression of the full-sequenced genes. With the testicular probe and a mixture of probes of 10 other tissues, 316 of 332 effective spots showed intense hybridized signals and 75 cDNAs were shown to be expressed very highly in the cynomolgus monkey testis, but not ubiquitously. Conclusions In this report, we determined 302 full-insert sequences of cynomolgus monkey cDNAs with enough length of open reading frames to discover novel transcripts as human homologues. Among 302 cDNA sequences, human homologues of 89 cDNAs have not been predicted in the annotated human genome sequence in the Ensembl. Additionally, we identified 75 dominantly expressed genes in testis among the full-sequenced clones by using a DNA microarray. Our cDNA clones and analytical results will be valuable resources for future functional genomic studies.
机译：背景为了有助于建立人类基因组转录区的完整图谱，我们为食蟹猴构建了一个睾丸cDNA文库，并试图寻找新颖的转录本来鉴定其人类同源物。结果确定了512个cDNA克隆的完整插入序列。最终，我们发现302个非冗余cDNA带有300 bp或更长的开放阅读框。其中，发现在Ensembl人类数据库中以前没有注释89个cDNA。在Ensembl小鼠数据库中搜索后，我们还发现Ensembl的带注释的人类和小鼠基因组序列中69个推定的编码序列没有同源cDNA。随后，我们设计了一个包含396个非冗余cDNA（有和没有开放阅读框）的DNA微阵列，以检查全序列基因的表达。使用睾丸探针和其他10个组织的探针混合物，在332个有效点中有316个显示了强烈的杂交信号，并且显示75个cDNA在食蟹猴睾丸中非常高地表达，但并不普遍存在。结论在本报告中，我们确定了302个食蟹猴cDNA的全插入序列，并具有足够的开放阅读框长度，以发现新的转录本作为人类同源物。在302个cDNA序列中，尚未在Ensembl中带注释的人类基因组序列中预测89个cDNA的人类同源物。此外，我们通过使用DNA微阵列在全序列克隆中的睾丸中鉴定了75个显性表达的基因。我们的cDNA克隆和分析结果将为将来的功能基因组研究提供宝贵的资源。

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