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首页> 外文期刊>Applied and Environmental Microbiology >Dichloran as an Inhibitor of Mold Spreading in Fungal Plating Media: Effects on Colony Diameter and Enumeration
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Dichloran as an Inhibitor of Mold Spreading in Fungal Plating Media: Effects on Colony Diameter and Enumeration

机译：敌百虫作为霉菌在真菌电镀介质中扩散的抑制剂：对菌落直径和枚举的影响

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摘要

Problems associated with overgrowth by spreading molds are not addressed by currently recommended fungal enumeration media. Twenty-two fungi, including 12 mold and 3 yeast genera, were evaluated for the effects of dichloran (2,6-dichloro-4-nitroaniline), previously identified as a mold-spreading inhibitor, on colony diameter and enumeration. On malt agar or antibiotic-potato-dextrose agar (APDA), colony diameters were effectively reduced when dichloran was added. Colony diameters decreased as the dichloran concentration increased. Counts obtained with mixed mold spore suspensions were lower on APDA supplemented with 25 μg of dichloran per ml than on APDA and were higher than APDA with the addition of 5 μg of dichloran per ml (APDA-D-5). Overall counts of mixed and individual mold spore and yeast suspensions were higher in APDA-D-5 than in APDA. The additional advantages of APDA-D-5 may be useful in routine enumeration of fungi.
机译：当前推荐的真菌计数介质不能解决因霉菌扩散而引起的过度生长的问题。评价了十二种霉菌（包括12个霉菌和3个酵母属）对事先确定为霉菌传播抑制剂的敌百虫（2,6-dichloro-4-nitroaniline）对菌落直径和计数的影响。在麦芽琼脂或抗生素-马铃薯-葡萄糖琼脂（APDA）上，加入二氯甲烷可有效减少菌落直径。菌落直径随二氯甲烷浓度的增加而减小。混合霉菌孢子悬浮液获得的计数在每毫升补充有25μg敌百虫每毫升的APDA上比在APDA上低，并且比每毫升加入5μg的敌百虫（APDA-D-5）更高。 APDA-D-5的混合和单个霉菌孢子和酵母悬浮液的总计数高于APDA。 APDA-D-5的其他优点可能在常规真菌计数中有用。

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