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首页> 外文期刊>Milchwissenschaft >Effects of suspension-dilution buffers and plating media on enumeration of Bifidobacterium.
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Effects of suspension-dilution buffers and plating media on enumeration of Bifidobacterium.

机译：悬浮液稀释缓冲液和平板培养基对双歧杆菌计数的影响。

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The effects of suspension-dilution buffers and plating media on viable counts of bifidobacteria were examined using 8 Bifidobacterium spp. Although the effects of suspension-dilution buffers on viable counts were significantly different in many species, Mitsuoka's buffer, originally developed for anaerobic intestinal microflora, was useful for most Bifidobacterium spp. Also, reinforced clostridia agar medium was more suitable than the other media tested. When 9 commercial bifidobacteria products (probiotic ingredients, dried milk, fermented milk and yoghurt) were analysed to confirm the aforementioned results, the same trend was observed. Thus, it is suggested that the selection of suspension-dilution buffer is very important for the enumeration of bifidobacteria and that Mitsuoka's buffer is a strong candidates for use in the development of a standard enumeration method.
机译：使用8个双歧杆菌属菌种检测了悬浮液稀释缓冲液和平板培养基对双歧杆菌活菌计数的影响。尽管悬浮稀释缓冲液对活菌计数的影响在许多物种中都存在显着差异，但最初为厌氧肠道菌群开发的Mitsuoka缓冲液可用于大多数双歧杆菌属。同样，强化梭状芽孢杆菌琼脂培养基比其他测试培养基更合适。当分析9种商用双歧杆菌产品（益生菌成分，奶粉，发酵奶和酸奶）以确认上述结果时，观察到相同的趋势。因此，建议悬浮稀释缓冲液的选择对于双歧杆菌的计数非常重要，而Mitsuoka的缓冲液是开发标准计数方法的强有力的候选者。

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