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首页> 外文期刊>Applied and Environmental Microbiology >Enzyme immunoassay for macrolide antibiotics: characterization of an antibody to 23-amino-O-mycaminosyltylonolide.
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Enzyme immunoassay for macrolide antibiotics: characterization of an antibody to 23-amino-O-mycaminosyltylonolide.

机译：大环内酯类抗生素的酶免疫测定：23-氨基-O-mycaminosyltylonolide抗体的表征。

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摘要

An enzyme-linked immunosorbent assay was developed for the detection of macrolide antibiotics by using a polyclonal antibody generated in rabbits immunized with 23-amino-O-mycaminosyltylonolide (23-amino-OMT) covalently linked to keyhole limpet hemocyanin. The specificity and sensitivity of this antibody were characterized by using 23-amino-OMT coupled to alkaline phosphatase as an enzyme-linked label in a direct competitive enzyme-linked immunosorbent assay. The assay sensitivity was as low as 0.3 ng/ml for 23-amino-OMT, with a 50% inhibitory concentration of 8 ng/ml. This antibody exhibited good reactivity with 12-, 14- or 16-membered macrolides possessing amino-substituted sugar moieties, regardless of the presence of neutral sugar residues. Little or no cross-reactivity was observed with the macrocyclic lactone ring structure (tylactone) or macrolides containing only neutral sugars. No cross-reaction was observed with polyenes or nonmacrolide antibiotics. Known macrolide-producing cultures grown in fermentation broth also showed good reactivity, indicating that this assay is useful in detecting this class of metabolites in fermentation.
机译：开发了一种酶联免疫吸附测定法，通过使用在兔体内产生的多克隆抗体来检测大环内酯类抗生素，所述兔用与匙孔血蓝蛋白共价连接的23-氨基-O-mycaminosyltylonolide（23-氨基-OMT）进行免疫。通过在直接竞争性酶联免疫吸附试验中使用与碱性磷酸酶偶联的23-氨基-OMT作为酶联标记来表征该抗体的特异性和敏感性。对于23-氨基-OMT，测定灵敏度低至0.3 ng / ml，50％抑制浓度为8 ng / ml。该抗体与具有氨基取代的糖部分的12、14或16元大环内酯类药物表现出良好的反应性，而与中性糖残基的存在无关。大环内酯环结构（内酯）或仅含有中性糖的大环内酯类几乎没有观察到交叉反应。与多烯或非大环内酯抗生素未观察到交叉反应。在发酵液中生长的已知产生大环内酯的培养物也显示出良好的反应性，表明该测定法可用于检测发酵中的此类代谢物。

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