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首页> 外文期刊>Applied and Environmental Microbiology >Inhibition of Epsilon-Poly-l-Lysine Biosynthesis in Streptomycetaceae Bacteria by Short-Chain Polyols
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Inhibition of Epsilon-Poly-l-Lysine Biosynthesis in Streptomycetaceae Bacteria by Short-Chain Polyols

机译：短链多元醇对链霉菌科细菌中Epsilon-Poly-1-赖氨酸生物合成的抑制作用

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Antimicrobial epsilon-poly-l-lysine (ePL) is secreted by Streptomycetaceae bacteria, and the mechanism of ePL biosynthesis remains to be elucidated. We previously reported that an unknown ePL derivative accumulates in the culture medium of ePL-producing bacteria when glycerol is added to the culture medium (Nishikawa and Ogawa, Appl. Environ. Microbiol. 68:3575-3581, 2002). In this study, by using matrix-assisted laser desorption ionization—time of flight mass spectrometry and nuclear magnetic resonance, we identified the unknown derivative as the ester formed between the hydroxyl group of a glycerol molecule and the terminal carboxyl group of an ePL molecule. When a short-chain aliphatic polyol, such as ethylene glycol, propanediol, or butanediol, was added instead of glycerol, a corresponding ePL-polyol monoester accumulated in the culture medium of ePL-producing bacteria. ePL esterification was accompanied by ePL synthesis in intact cells and a cell-free system, but no esterification of exogenous ePL was observed. ePL-polyol esters were formed during lysine polymerization. The number of lysine residues of ePL-polyol esters decreased with increasing polyol concentration. Taken together, these results indicate that ePL synthesis is inhibited by polyols via esterification and that ePL elongation occurs via the incorporation of lysine monomers into the carboxyl terminus of ePL.
机译：链霉菌细菌分泌了抗菌素ε-聚赖氨酸（ePL），ePL生物合成的机理尚待阐明。我们先前曾报道，当将甘油添加到培养基中时，未知的ePL衍生物会在产生ePL的细菌的培养基中积累（Nishikawa和Ogawa，Appl。Environ。Microbiol。68：3575-3581，2002）。在这项研究中，通过使用基质辅助激光解吸电离—飞行时间质谱和核磁共振，我们确定了未知衍生物为甘油分子的羟基与ePL分子的末端羧基之间形成的酯。当添加诸如乙二醇，丙二醇或丁二醇的短链脂族多元醇代替甘油时，相应的ePL-多元醇单酯累积在产生ePL的细菌的培养基中。 ePL酯化伴随完整细胞和无细胞系统中ePL的合成，但未观察到外源性ePL的酯化。 ePL-多元醇酯是在赖氨酸聚合过程中形成的。 ePL-多元醇酯的赖氨酸残基数量随着多元醇浓度的增加而降低。总之，这些结果表明，多元醇通过酯化作用抑制了ePL的合成，并且通过将赖氨酸单体掺入ePL的羧基末端而发生了ePL的延伸。

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