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首页> 外文期刊>Applied and Environmental Microbiology >Characterization of Antimicrobial Substances Produced by Enterococcus faecalis MRR 10-3, Isolated from the Uropygial Gland of the Hoopoe (Upupa epops)
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Characterization of Antimicrobial Substances Produced by Enterococcus faecalis MRR 10-3, Isolated from the Uropygial Gland of the Hoopoe (Upupa epops)

机译：粪肠球菌MRR 10-3产生的抗菌物质的特征，从戴胜的戴比尔腺体分离（Upupa epops）

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The uropygial gland (preen gland) is a holocrine secretory gland situated at the base of the tail in birds which produces a hydrophobic fatty secretion. In certain birds, such as the hoopoe, Upupa epops, the composition of this secretion is influenced by both seasonal and sexual factors, becoming darker and more malodorous in females and in their nestlings during the nesting phase. The secretion is spread throughout the plumage when the bird preens itself, leaving its feathers flexible and waterproof. It is also thought to play a role in defending the bird against predators and parasites. We have isolated from the uropygial secretion of a nestling a bacterium that grows in monospecific culture which we have identified unambiguously by phenotypic and genotypic means as Enterococcus faecalis. The strain in question produces antibacterial substances that are active against all gram-positive bacteria assayed and also against some gram-negative strains. Its peptide nature identifies it as a bacteriocin within the group known as enterocins. Two peptides were purified to homogeneity (MR10A and MR10B), and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) analysis showed masses of 5201.58 and 5207.7 Da, respectively. Amino acid sequencing of both peptides revealed high similarity with enterocin L50A and L50B (L. M. Cintas, P. Casaus, H. Holo, P. E. Hernández, I. F. Nes, and L. S. H?varstein, J. Bacteriol. 180:1988-1994, 1998). PCR amplification of total DNA from strain MRR10-3 with primers for the L50A/B structural genes and sequencing of the amplified fragment revealed almost identical sequences, except for a single conservative change in residue 38 (Glu→Asp) in MR10A and two changes in residues 9 (Thr→Ala) and 15 (Leu→Phe) in MR10B. This is the first time that the production of bacteriocins by a bacterium isolated from the uropygial gland has been described. The production of these broad-spectrum antibacterial substances by an enterococcal strain living in the uropygial gland may be important to the hygiene of the nest and thus to the health of the eggs and chicks.
机译：尿py腺（preen gland）是位于鸟尾巴底部的全分泌腺，会产生疏水性脂肪分泌。在某些鸟类中，例如戴胜鸟，Upupa epops，这种分泌物的成分受季节和性因素的影响，在筑巢阶段雌性及其雏鸟中的深色和恶臭程度更高。当鸟类自夸时，分泌物会散布在整个羽毛中，从而使其羽毛柔韧而防水。人们还认为它在保护鸟类免受掠食者和寄生虫的侵害中发挥了作用。我们已从雏鸟的尿道泌尿分泌物中分离出一种在单特异性培养物中生长的细菌，我们已通过表型和基因型方法明确地将其鉴定为粪肠球菌。所讨论的菌株产生的抗菌物质对所有测定的革兰氏阳性细菌以及某些革兰氏阴性菌株均具有活性。它的肽性质将其识别为细菌，称为肠毒素。将两种肽纯化至均一（MR10A和MR10B），基质辅助激光解吸电离飞行时间（质谱）分析的质量分别为5201.58和5207.7 Da。两种肽的氨基酸测序都显示与肠球蛋白L50A和L50B高度相似（LM Cintas，P.Casaus，H.Holo，PEHernández，IF Nes，和LS H？varstein，J.Bacteriol。180：1988-1994，1998） 。用L50A / B结构基因的引物对MRR10-3菌株的总DNA进行PCR扩增，并对扩增片段进行测序，发现序列几乎相同，除了MR10A中第38位残基的一个保守变化（Glu→Asp）和两个MR10B中的9（Thr→Ala）和15（Leu→Phe）残基。这是首次描述了从尿道腺分离的细菌产生细菌素的过程。生活在尿道腺中的肠球菌菌株产生这些广谱抗菌物质，可能对产蛋箱的卫生以及鸡蛋和雏鸡的健康很重要。

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