Purification of Large Amounts of Murine Ribonucleic Acid Tumor Viruses Produced in Roller Bottle Cultures

James J. Syrewicz; R. B. Naso; C. S. Wang; Ralph B. Arlinghaus

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Purification of Large Amounts of Murine Ribonucleic Acid Tumor Viruses Produced in Roller Bottle Cultures

机译：滚瓶培养物中产生的大量鼠核糖核酸肿瘤病毒的纯化

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摘要

Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.

机译：鼠核糖核酸肿瘤病毒和C型病毒颗粒在滚瓶培养中产生相对大量。可以通过简单的两步程序纯化存在于大量培养液中的病毒，该程序包括聚乙二醇沉淀和蔗糖密度梯度的平衡离心。

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《Applied Microbiology》 |1972年第3期|共7页
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James J. Syrewicz; R. B. Naso; C. S. Wang; Ralph B. Arlinghaus;
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中图分类微生物学;
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1. Large-Scale Purification of Ribonucleic Acid Tumor Viruses by Use of Continuous-Flow Density Gradient Centrifugation [J] . D. P. Grandgenett, K. Brackmann, M. Green Applied Microbiology . 1973,第3期

机译：通过连续流密度梯度离心大规模纯化核糖核酸肿瘤病毒
2. Deoxyribonucleic acid-dependent ribonucleic acid polymerases from murine spleen cells. Increased amounts of the nucleolar species in leukaemic tissue [J] . Donner F. Babcock, Marvin A. Rich The biochemical journal . 1973,第4期

机译：来自鼠脾细胞的脱氧核糖核酸依赖性核糖核酸聚合酶。白血病组织中核仁种类的数量增加
3. Mechanism of Viral Carcinogenesis by Deoxyribonucleic Acid Mammalian Viruses IV. Related Virus-specific Ribonucleic Acids in Tumor Cells Induced by “Highly” Oncogenic Adenovirus Types 12, 18, and 31 [J] . Kei Fujinaga, Maurice Green Journal of Virology . 1967,第3期

机译：脱氧核糖核酸哺乳动物病毒IV的病毒致癌机制。肿瘤细胞中的相关病毒特异性核糖核酸，其“高度”致癌腺病毒12,18和31型
4. Simulation analysis and experiment of unpowered roller conveying for culture bottle in tissue culture plant [C] . Yanli Yang, Qi Chu, Meizhang Gu, International Conference on Automation, Control and Robotics Engineering . 2021

机译：组织培养厂培养瓶输送无动力轧辊的仿真分析及实验
5. Therapeutic vaccination with ribonucleic acids encoding tumor/self antigens and a cytokine adjuvant in a transgenic murine model of prostatic carcinoma. [D] . Hess, Paul Robert. 2002

机译：在前列腺癌的转基因鼠模型中，用编码肿瘤/自身抗原的核糖核酸和细胞因子佐剂进行治疗性疫苗接种。
6. Purification of Large Amounts of Murine Ribonucleic Acid Tumor Viruses Produced in Roller Bottle Cultures [O] . James J. Syrewicz, R. B. Naso, C. S. Wang, 1972

机译：滚瓶培养物中产生的大量鼠核糖核酸肿瘤病毒的纯化
7. Purification of Large Amounts of Murine Ribonucleic Acid Tumor Viruses Produced in Roller Bottle Cultures [O] . James J. Syrewicz, R. B. Naso, C. S. Wang, 1972

机译：辊瓶培养生产大量鼠核糖核酸肿瘤病毒的纯化

Purification of Large Amounts of Murine Ribonucleic Acid Tumor Viruses Produced in Roller Bottle Cultures

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