• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Determination of Endotoxicity in Bacterial Vaccines
【24h】

Determination of Endotoxicity in Bacterial Vaccines

机译：细菌疫苗内毒素的测定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endotoxicity of bacterial vaccines was quantitated in mice by using actinomycin D as potentiating agent. The results were compared with those obtained by the mouse weight gain test. The lethality of Escherichia coli lipopolysaccharide was increased 2,140 times when 12.5 μg of actinomycin D was used. The mean lethal dose values of heated and unheated pertussis vaccines were similar in the actinomycin D enhancement assay, but the unheated vaccine was significantly more toxic in the mouse weight gain test. Acetone-inactivated typhoid vaccine was slightly less toxic than the heat-phenol-inactivated vaccine in both the actinomycin D enhancement assay and mouse weight gain test. Endotoxicity of experimental vaccines prepared by extraction of Pseudomonas aeruginosa strains was high as compared with E. coli lipopolysaccharide. The BALB/c mice were about four times more susceptible than the random bred NIH strain mice. The results indicate that the actinomycin D enhancement assay had the advantages of being more sensitive and probably more specific.
机译：通过使用放线菌素D作为增强剂对细菌疫苗的内毒性进行了定量。将结果与通过小鼠体重增加测试获得的结果进行比较。当使用12.5μg放线菌素D时，大肠杆菌脂多糖的致死性提高了2140倍。在放线菌素D增强试验中，加热和未加热的百日咳疫苗的平均致死剂量值相似，但在小鼠体重增加试验中，未加热的疫苗的毒性更大。在放线菌素D增强试验和小鼠体重增加试验中，丙酮灭活伤寒疫苗的毒性比热酚灭活疫苗稍低。与铜绿假单胞菌脂多糖相比，通过提取铜绿假单胞菌菌株制备的实验疫苗的内毒性较高。 BALB / c小鼠的敏感度是随机繁殖的NIH品系小鼠的四倍。结果表明放线菌素D增强测定法具有更灵敏且可能更特异性的优点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号