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首页> 外文期刊>Applied Microbiology >Distribution and effects of 2,4,5-trichlorophenoxyacetic acid in cells of Bacillus megaterium.
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Distribution and effects of 2,4,5-trichlorophenoxyacetic acid in cells of Bacillus megaterium.

机译：2,4,5-三氯苯氧基乙酸在巨大芽孢杆菌细胞中的分布及其作用。

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摘要

Cell death in a resting population of an asporogenous Bacillus megaterium was accelerated by ambient concentrations of 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) equal to or greater than 10 mug/ml or 5 mug/mg of cells (dry weight), but only after prolonged exposure. Conversely, populations of growing cells were not markedly influenced even at 100 mug/ml. Effects on cell respiration were not manifest until the ambient concentration reached 1,000 mug of 2,4,5-T/ml, or 500 mug/mg. Cells of B. megaterium did, however, accumulate 2,4,5-T passively to a level approximately twofold above the ambient concentration. Most of the accumulated compound was easily washed from the cells, but, of the firmly bound herbicide, about 0.5 mug/mg of cells (dry weight), nearly 60% by weight, was localized in the protoplast membrane. The foregoing results, obtained with a purified preparation of 2,4,5-T were also elicited by 2,4,5-T analytical standards. The extracted contaminants did not produce the results alone nor did they influence the results when present in combination with 2,4,5-T.
机译：环境浓度等于或大于10杯/毫升或5杯/毫克细胞的2,4,5-三氯苯氧基乙酸（2,4,5-T）的环境浓度加速了静止的巨大孢子芽孢杆菌种群中的细胞死亡（干重），但仅在长时间暴露后才能使用。相反，即使浓度为100杯/毫升，生长细胞的数量也没有受到明显影响。直到环境浓度达到1,000杯2,4,5-T / ml或500杯/ mg时，对细胞呼吸的影响才显现出来。然而，巨大芽孢杆菌的细胞确实被动积累了2,4,5-T，其浓度约为环境浓度的两倍。大部分积聚的化合物很容易从细胞中洗去，但是，牢固结合的除草剂中约有0.5马克/毫克的细胞（干重）接近60％（重量）位于原生质体膜中。用2,4,5-T分析标准品也可以得出纯化的2,4,5-T制剂获得的上述结果。当与2,4,5-T组合使用时，提取的污染物既不能单独产生结果，也不会影响结果。

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