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首页> 外文期刊>Applied and Environmental Microbiology >Strain-Specific Genotyping of Bifidobacterium animalis subsp. lactis by Using Single-Nucleotide Polymorphisms, Insertions, and Deletions
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Strain-Specific Genotyping of Bifidobacterium animalis subsp. lactis by Using Single-Nucleotide Polymorphisms, Insertions, and Deletions

机译：动物双歧杆菌亚种的菌株特异性基因分型通过使用单核苷酸多态性，插入和删除乳酸

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Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics demands techniques with high discriminatory power to differentiate among strains. However, to date, molecular approaches, such as pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR, have been ineffective at achieving strain separation due to the monomorphic nature of this subspecies. Previously, sequencing and comparison of two B. animalis subsp. lactis genomes (DSMZ 10140 and Bl-04) confirmed this high level of sequence similarity, identifying only 47 single-nucleotide polymorphisms (SNPs) and four insertions and/or deletions (INDELs) between them. In this study, we hypothesized that a sequence-based typing method targeting these loci would permit greater discrimination between strains than previously attempted methods. Sequencing 50 of these loci in 24 strains of B. animalis subsp. lactis revealed that a combination of nine SNPs/INDELs could be used to differentiate strains into 14 distinct genotypic groups. In addition, the presence of a nonsynonymous SNP within the gene encoding a putative glucose uptake protein was found to correlate with the ability of certain strains to transport glucose and to grow rapidly in a medium containing glucose as the sole carbon source. The method reported here can be used in clinical, regulatory, and commercial applications requiring identification of B. animalis subsp. lactis at the strain level.
机译：动物双歧杆菌亚种的几种益生菌菌株。乳酸菌由于其已证明的健康益处以及在哺乳动物胃肠道和酸化乳制品中生存的能力而被广泛地添加到食品和膳食补充剂中。这些特性的菌株特异性要求具有高区分能力的技术以区分菌株。然而，迄今为止，由于该亚种的单态性，分子方法，例如脉冲场凝胶电泳和随机扩增的多态性DNA-PCR，在实现菌株分离方面一直无效。以前，对两个动物双歧杆菌亚种进行测序和比较。乳酸基因组（DSMZ 10140和Bl-04）证实了这种高水平的序列相似性，仅鉴定出47个单核苷酸多态性（SNP）以及它们之间的四个插入和/或缺失（INDEL）。在这项研究中，我们假设针对这些基因座的基于序列的分型方法比以前尝试的方法在菌株之间具有更大的区分度。在24个动物双歧杆菌亚种中对这些基因座中的50个进行测序。乳酸揭示了九种SNP / INDEL的组合可用于将菌株区分为14个不同的基因型组。另外，发现在编码推定的葡萄糖摄取蛋白的基因中存在非同义SNP与某些菌株在包含葡萄糖作为唯一碳源的培养基中运输葡萄糖并快速生长的能力相关。本文报道的方法可用于需要鉴定动物双歧杆菌亚种的临床，法规和商业应用。乳酸菌在菌株水平。

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