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首页> 外文期刊>Applied Microbiology >Influence of the Plant Defense Response to Escherichia coli O157:H7 Cell Surface Structures on Survival of That Enteric Pathogen on Plant Surfaces
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Influence of the Plant Defense Response to Escherichia coli O157:H7 Cell Surface Structures on Survival of That Enteric Pathogen on Plant Surfaces

机译：植物防御反应对大肠杆菌O157：H7细胞表面结构的影响对肠道病原体在植物表面存活的影响

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Consumption of fresh and fresh-cut fruits and vegetables contaminated with Escherichia coli O157:H7 has resulted in hundreds of cases of illness and, in some instances, death. In this study, the influence of cell surface structures of E. coli O157:H7, such as flagella, curli fimbriae, lipopolysaccharides, or exopolysaccharides, on plant defense responses and on survival or colonization on the plant was investigated. The population of the E. coli O157:H7 ATCC 43895 wild-type strain was significantly lower on wild-type Arabidopsis plants than that of the 43895 flagellum-deficient mutant. The population of the E. coli O157:H7 43895 flagellum mutant was greater on both wild-type and npr1-1 mutant (nonexpressor of pathogenesis-related [PR] genes) plants and resulted in less PR gene induction, estimated based on a weak β-glucuronidase (GUS) signal, than did the 43895 wild-type strain. These results suggest that the flagella, among the other pathogen-associated molecular patterns (PAMPs), made a substantial contribution to the induction of plant defense response and contributed to the decreased numbers of the E. coli O157:H7 ATCC 43895 wild-type strain on the wild-type Arabidopsis plant. A curli-deficient E. coli O157:H7 86-24 strain survived better on wild-type Arabidopsis plants than the curli-producing wild-type 86-24 strain did. The curli-deficient E. coli O157:H7 86-24 strain exhibited a GUS signal at a level substantially lower than that of the curli-producing wild-type strain. Curli were recognized by plant defense systems, consequently affecting bacterial survival. The cell surface structures of E. coli O157:H7 have a significant impact on the induction of differential plant defense responses, thereby impacting persistence or survival of the pathogen on plants.
机译：食用被O157：H7大肠杆菌污染的新鲜水果和鲜切水果和蔬菜已导致数百例疾病，甚至在某些情况下导致死亡。在这项研究中，研究了大肠杆菌O157：H7的细胞表面结构（如鞭毛，卷曲菌，脂多糖或胞外多糖）对植物防御反应以及对植物存活或定植的影响。与野生型拟南芥植物相比，大肠杆菌O157：H7 ATCC 43895野生型菌株的种群明显低于鞭毛缺陷型43895。大肠杆菌O157：H7 43895鞭毛突变体的种群在野生型和npr1-1突变体（致病相关[PR]基因的非表达子）植株上均较大，并且基于弱与43895野生型菌株相比，β-葡萄糖醛酸苷酶（GUS）信号更高。这些结果表明，鞭毛以及其他病原体相关的分子模式（PAMPs），对诱导植物防御反应做出了重要贡献，并导致大肠杆菌O157：H7 ATCC 43895野生型菌株数量减少在野生型拟南芥植物上。缺乏卷曲的大肠杆菌O157：H7 86-24菌株在野生型拟南芥植物上的存活率比生产卷曲的野生型86-24菌株更好。缺乏卷曲的大肠杆菌O157：H7 86-24菌株表现出的GUS信号水平显着低于产生卷曲的野生型菌株的水平。 Curli被植物防御系统所认可，因此影响细菌的存活。大肠杆菌O157：H7的细胞表面结构对诱导不同的植物防御反应具有重要影响，从而影响病原体在植物上的持久性或存活性。

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