Candida utilis, Aureobasidium pullulans, and Trichoderma viride were grown on pretreated ryegrass straw. The pretreatment consisted of hydrolysis of straw with 0.5 N H2SO4 (water-substrate, 3:1) at 121 C, 100 C, and room temperature and adjustment of the hydrolysate to pH 4.5 to 5.0 with 5 N NH4OH. The 121 C pretreatment yielded a material containing 30% sugar and 2.3% N. The fermentation was carried on semisolid substrate (moisture level, 75%) in rotating jars for 2 to 3 days at room temperature. The organisms grew rapidly during the period from 18 to 42 h of incubation. During this period the number of microbial cells increased by 20- to 200-fold, and the level of NH3-N decreased from 1.3 to 0.9%. The fermentation resulted in a fourfold increase in protein, fivefold increase in crude fat, and 40% increase in the digestibility of straw. The best result in terms of increasing protein and digestibility of straw was obtained when C. utilis was grown on straw preheated at 121 C.
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机译：在经过处理的黑麦草秸秆上种植了假丝酵母，金黄色葡萄球菌和木霉。预处理包括在121°C，100°C和室温下用0.5 N H2SO4（水为基质，3：1）和室温下的稻草进行水解，然后用5 N NH4OH将水解液的pH值调节至4.5至5.0。 121 C预处理产生的物质包含30％的糖和2.3％的氮。发酵在半固态底物（水分含量为75％）上在旋转罐中于室温进行2-3天。在培养18至42小时的过程中，这些生物迅速生长。在此期间，微生物细胞数量增加了20到200倍，而NH3-N的含量从1.3降低到0.9％。发酵使蛋白质增加了4倍，粗脂肪增加了5倍，秸秆的消化率提高了40％。当将鱼C. utilis生长在预热到121°C的秸秆上时，就可以提高秸秆的蛋白质和消化率，这是最好的结果。
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