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首页> 外文期刊>Applied Microbiology >Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch
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Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch

机译：涉及控制霍乱弧菌粘附于颗粒淀粉的机制

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Vibrio cholerae has been shown to adhere to cornstarch granules. The present work explored the mechanisms involved in this adhesion and the possibility of its occurrence in vivo . The findings suggest that both specific and nonspecific interactions are involved in the adhesion. Nonspecific hydrophobic interactions may play a role, since both V. cholerae and cornstarch granules exhibited hydrophobic properties when they were tested using a xylene-water system. In addition, the presence of bile acids reduced the adhesion. The adhesion also involves some specific carbohydrate-binding moieties on the cell surface, as reflected by reduced adhesion following pretreatment of the bacteria with proteinase K and sodium m -periodate. Further investigations supported these observations and showed that media containing low-molecular-weight carbohydrates had a significant inhibitory effect. Binding cell lysate to starch granules and removing the adhered proteins using either glucose or bile acids led to identification (by liquid chromatography-tandem mass spectrometry analysis) of several candidate V. cholerae outer membrane-associated starch-binding proteins. Different sets of proteins were isolated by removal in a glucose solution or bile acids. When the upper gastrointestinal tract conditions were simulated in vitro , both bile salts and the amylolytic activity of the pancreatic juices were found to have an inhibitory effect on the adherence of V. cholerae to starch. However, during acute diarrhea, this inhibitory effect may be significantly reduced due to dilution, suggesting that adhesion does occur in vivo . Such adhesion may contribute to the beneficial effects observed following administration of granular starch-based oral rehydration solutions to cholera patients.
机译：霍乱弧菌已显示粘附在玉米淀粉颗粒上。目前的工作探索了这种黏附的机制及其在体内发生的可能性。该发现表明粘附中既涉及特异性相互作用又涉及非特异性相互作用。非特异性疏水相互作用可能起一定作用，因为霍乱弧菌和玉米淀粉颗粒在使用二甲苯-水系统进行测试时均表现出疏水特性。另外，胆汁酸的存在降低了粘附力。粘附还涉及细胞表面上的一些特定的碳水化合物结合部分，这通过用蛋白酶K和间高碘酸钠预处理细菌后粘附减少而反映出来。进一步的研究支持了这些观察结果，并表明含有低分子量碳水化合物的培养基具有明显的抑制作用。将细胞裂解物与淀粉颗粒结合，并使用葡萄糖或胆汁酸去除粘附的蛋白质，从而鉴定（通过液相色谱-串联质谱分析）几种霍乱弧菌外膜相关淀粉结合蛋白。通过在葡萄糖溶液或胆汁酸中去除来分离不同组的蛋白质。当在体外模拟上胃肠道条件时，发现胆汁盐和胰液的淀粉分解活性均对霍乱弧菌对淀粉的粘附具有抑制作用。但是，在急性腹泻期间，由于稀释作用，这种抑制作用可能会大大降低，表明在体内确实发生了粘附。在向霍乱患者施用基于淀粉的颗粒状口服补液后，这种粘附可能有助于观察到有益的效果。

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