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首页> 外文期刊>Applied Microbiology >Rapid Methods for Extracting Autolysins from Bacillus subtilis
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Rapid Methods for Extracting Autolysins from Bacillus subtilis

机译：从枯草芽孢杆菌中提取自溶素的快速方法

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Two procedures are described for the extraction of autolysins from whole cells. One method uses 5 M LiCl at 4 C. The amount of enzyme obtained by this method is six times more than that obtained by autolysis of cell walls and fourteen times more than that obtained by extracting cell walls with LiCl. With the other method, cells are extracted with 2% Triton X-100. This is less efficient than the LiCl method but yields about one-half the amount of enzyme obtained by cell wall autolysis and about the same amount as obtained by extracting cell walls with salt. Both procedures yield autolysin with multiple pH optima. Autolysins can be extracted from several bacterial species by either the LiCl or the detergent method. The data suggest that these techniques have sufficient sensitivity to detect small differences in autolytic activity among mutants and various organisms and are also suitable for large-scale isolation of autolysin for purification and characterization studies.
机译：描述了两种从全细胞中提取自溶素的方法。一种方法是在4 C下使用5 M LiCl。通过这种方法获得的酶量是通过细胞壁自溶作用获得的酶的六倍，是通过用LiCl提取细胞壁获得的酶的十四倍。用另一种方法，用2％Triton X-100提取细胞。它的效率不如LiCl方法，但产生的酶量大约是通过细胞壁自溶作用获得的酶量的一半，而产生的酶量却与用盐提取细胞壁获得的酶量相同。两种方法均产生具有多个最佳pH值的自溶素。可以通过LiCl或去污剂方法从几种细菌中提取自溶素。数据表明，这些技术具有足够的灵敏度，可以检测突变体和各种生物之间自溶活性的微小差异，并且还适合大规模分离自溶素以进行纯化和表征研究。

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